Datta S, Datta S C
J Assoc Off Anal Chem. 1976 Nov;59(6):1255-7.
A rapid method for the estimation of available lysine in dried milk powder has been developed. Samples were treated with 2,4,6-trinitrobenzene sulfonic acid and then hydrolyzed with 6N HCl. The hydrolysate was purified and subjected to ascending thin layer chromatography to isolate xi-trinitophenyl lysine in pure form. The color was read at 346 nm in a specitrophotometer.
已开发出一种快速估算奶粉中有效赖氨酸含量的方法。样品用2,4,6-三硝基苯磺酸处理，然后用6N盐酸水解。水解产物经过纯化，并进行上行薄层色谱分析以分离出纯形式的ε-三硝基苯赖氨酸。在分光光度计中于346nm处读取颜色。
