Long A R, Hsieh L C, Malbrough M S, Short C R, Barker S A
Louisiana State University, School of Veterinary Medicine, Department of Veterinary Physiology, Baton Rouge.
J Assoc Off Anal Chem. 1989 Sep-Oct;72(5):739-41.
A multiresidue method for the isolation and liquid chromatographic determination of 7 benzimidazole anthelmintics (thiabendazole, oxfendazole, para-hydroxyfenbendazole, fenbendazole sulfone, mebendazole, albendazole, and fenbendazole) in milk is presented. Blank or benzimidazole-spiked milk samples (0.5 mL) were blended with octadecylsilyl (C-18, 18% load, end-capped) derivatized silica packing material. A column made from the C-18/milk matrix was first washed with hexane (8 mL), and then the benzimidazoles were eluted with methylene chloride-ethyl acetate (1 + 2, v/v; 8 mL). The eluate contained benzimidazole analytes which were free from interfering compounds as determined by UV detection (photodiode array, 290 nm). Correlation coefficients of standard curves for individual benzimidazoles isolated from spiked samples were linear (0.989 +/- 0.003 to 0.998 +/- 0.001) with recoveries ranging from 70 +/- 9% to 107 +/- 2% for the concentration range (62.5-2000 ng/mL) examined. The inter-assay variabilities ranged from 4 +/- 1% to 9 +/- 7% with intra-assay variabilities of 3-6%.
本文介绍了一种用于分离和液相色谱测定牛奶中7种苯并咪唑驱虫剂（噻苯达唑、奥芬达唑、对羟基芬苯达唑、芬苯达唑砜、甲苯达唑、阿苯达唑和芬苯达唑）的多残留方法。空白或添加了苯并咪唑的牛奶样品（0.5 mL）与十八烷基硅烷（C-18，18%负载量，封端）衍生化硅胶填充材料混合。由C-18/牛奶基质制成的柱先用己烷（8 mL）冲洗，然后用二氯甲烷-乙酸乙酯（1 + 2，v/v；8 mL）洗脱苯并咪唑。通过紫外检测（光电二极管阵列，290 nm）确定，洗脱液中含有的苯并咪唑分析物无干扰化合物。从加标样品中分离出的各苯并咪唑标准曲线的相关系数呈线性（0.989±0.003至0.998±0.001），在所检测的浓度范围（62.5 - 2000 ng/mL）内，回收率为70±9%至107±2%。批间变异系数为4±1%至9±7%，批内变异系数为3 - 6%。
