Jacobson H R, Gross J B, Kawamura S, Waters J D, Kokko J P
J Clin Invest. 1976 Nov;58(5):1233-9. doi: 10.1172/JCI108577.
Cortical and outer medullary collecting duct segments were dissected from human kidneys and perfused in vitro. The transepithelial potential difference was measured and found to be lumen positive +6.8 +/- 0.6 mV (n= 20). This lumen-positive potential difference was inhibited by ouabain and furosemide but not by acetazolamide. Replacement of chloride in bath and perfusion fluids caused a reversible decrease of the potential difference to near zero. We conclude from these studies: (a) the lumen-positive potential difference is dependent upon the presence of chloride ion suggesting the existence of an active electrogenic chloride reabsorptive process in the human collecting duct and (b) it is possible to examine human renal physiology directly using in vitro microperfusion of tubule segments.
从人肾脏中分离出皮质和外髓集合管段，并在体外进行灌注。测量跨上皮电位差，发现管腔呈正电位，为+6.8±0.6 mV（n = 20）。这种管腔正电位差受到哇巴因和呋塞米的抑制，但不受乙酰唑胺的抑制。用氯化物替代浴液和灌注液中的氯化物会导致电位差可逆性降低至接近零。我们从这些研究中得出结论：（a）管腔正电位差取决于氯离子的存在，提示人集合管中存在主动电生成性氯离子重吸收过程；（b）使用肾小管段的体外微灌注直接研究人体肾脏生理学是可行的。
