Chiba A
Department of Biology, Nippon Dental University School of Dentistry at Niigata, Niigata, Japan.
Brain Behav Evol. 1999;53(2):102-9. doi: 10.1159/000006586.
The distribution of neuropeptide Y (NPY)-like immunoreactivity in the brain and hypophysis of the arctic lamprey, Lethenteron japonica, was studied by the use of polyclonal anti-synthetic porcine NPY antibody. Immunoreactivity was found throughout the brain, with varied densities among different areas. NPY-positive cell bodies were located in the dorsal pallium of the telencephalon; in the preoptic area, the thalamus, and the hypothalamus of the diencephalon; in the tegmentum of the mesencephalon; and in the octavolateralis nucleus, the middle reticular nucleus, and the vagal motor nucleus of the rhombencephalon. The majority of the NPY-positive cells in the thalamus and in the hypothalamus appeared as cerebrospinal fluid-contacting neurons. NPY-positive fibers were widely distributed in the brain and locally dense in the ventromedial part of the lateral pallium, in the posterior part of the interpeduncular nucleus, and in the raphe region. Conversely, they were less dense or well scattered in the olfactory bulb, the lateral pallium except for its ventromedial part, and the optic tectum. NPY-positive innervation in the neurohypophysis was evident, suggesting that involvement of NPY-related substance in the hypothalamo-hypophysial system had occurred in an early stage of vertebrate evolution.
利用抗合成猪神经肽Y（NPY）多克隆抗体，研究了北极七鳃鳗（Lethenteron japonica）脑和垂体中NPY样免疫反应性的分布。在整个脑中均发现有免疫反应性，不同区域的密度各不相同。NPY阳性细胞体位于端脑的背侧皮层；间脑的视前区、丘脑和下丘脑；中脑的被盖；以及后脑的八侧线核、中间网状核和迷走运动核。丘脑中的大多数NPY阳性细胞以及下丘脑中的大多数NPY阳性细胞表现为接触脑脊液的神经元。NPY阳性纤维广泛分布于脑中，在外侧皮层腹内侧部分、脚间核后部和中缝区域局部密集。相反，它们在嗅球、外侧皮层除腹内侧部分外以及视顶盖中的密度较低或分布较分散。神经垂体中的NPY阳性神经支配明显，这表明与NPY相关的物质在脊椎动物进化的早期阶段就已参与下丘脑 - 垂体系统。
