Lauf U, Müller C, Herrmann H
Institut für Genetik und Biochemie der Ernst-Moritz-Arndt-Universität Greifswald, Germany.
FEMS Microbiol Lett. 1999 Jan 15;170(2):407-12. doi: 10.1111/j.1574-6968.1999.tb13401.x.
A new insertion sequence (IS1383) was identified on plasmids from Pseudomonas putida strain H and its nucleotide sequence was determined. IS1383 contains perfect terminal inverted repeats of 13-bp flanking a 1.4-kb internal sequence. A single significant open reading frame was identified that can encode a 342-amino acid polypeptide which was predicted to be highly basic and to have homology to polypeptides known from several other bacterial insertion sequences. At least six copies of IS1383 are present on the plasmids pPGH1 and pPGH2, whereas no copy could be detected on the chromosome of P. putida strain H. Target duplications did not flank the inverted repeats of any of the six IS1383 copies examined. Analysis of the integration sites of IS1383 revealed hints for a target specificity. Multiple sequence alignments of the transposases, the inverted repeats and the integration sites pointed to the assignment of IS1383 into a putative new family of insertion sequences defined as the IS1111 family.
在恶臭假单胞菌H菌株的质粒上鉴定出一种新的插入序列（IS1383），并测定了其核苷酸序列。IS1383含有13个碱基对的完美末端反向重复序列，两侧是一个1.4千碱基的内部序列。鉴定出一个单一的重要开放阅读框，其可编码一个342个氨基酸的多肽，预测该多肽具有高度碱性，并且与其他几种细菌插入序列中已知的多肽具有同源性。在质粒pPGH1和pPGH2上至少存在六个IS1383拷贝，而在恶臭假单胞菌H菌株的染色体上未检测到任何拷贝。在所检测的六个IS1383拷贝中，没有任何一个的反向重复序列两侧存在靶标重复序列。对IS1383整合位点的分析揭示了靶标特异性的线索。转座酶、反向重复序列和整合位点的多序列比对表明，IS1383可归入一个假定的新插入序列家族，即IS1111家族。
