与其他细菌插入序列相关的1275个碱基对序列IS1246的两个相同拷贝，将TOL质粒pWW0上的木糖基因包围起来。
Two identical copies of IS1246, a 1275 base pair sequence related to other bacterial insertion sequences, enclose the xyl genes on TOL plasmid pWW0.
作者信息
Reddy B R, Shaw L E, Sayers J R, Williams P A
机构信息
School of Biological Sciences, University of Wales, Bangor, UK.
出版信息
Microbiology (Reading). 1994 Sep;140 ( Pt 9):2305-7. doi: 10.1099/13500872-140-9-2305.
Two identical direct repeats of a 1275 bp sequence, designated IS1246, encompass the xyl genes, which determine the catabolism of toluene, m- and p-xylenes to central metabolites, on the TOL catabolic plasmid pWW0. IS1246 has a terminal inverted repeat of 12 bp (5'GGGCACCTCGAA3') and contains a major open reading frame of 280 codons. This ORF shows significant homology with ORFs encoded by a number of bacterial insertion sequences from Bacteroides, Neisseria and Escherichia coli.
一段1275bp的序列（命名为IS1246）的两个相同的正向重复序列，环绕着木糖基因，这些基因决定了TOL分解代谢质粒pWW0上甲苯、间二甲苯和对二甲苯向中心代谢物的分解代谢。IS1246有一个12bp的末端反向重复序列（5'GGGCACCTCGAA3'），并包含一个由280个密码子组成的主要开放阅读框。这个开放阅读框与来自拟杆菌属、奈瑟菌属和大肠杆菌的许多细菌插入序列所编码的开放阅读框具有显著的同源性。
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