Asano S, Honda T, Goshima F, Watanabe D, Miyake Y, Sugiura Y, Nishiyama Y
J Gen Virol. 1999 Jan;80 ( Pt 1):51-56. doi: 10.1099/0022-1317-80-1-51.
To clarify the biological role of US3 protein kinase of herpes simplex virus type 2 (HSV-2) in vivo, the expression of the viral antigen, the appearance of apoptotic bodies and DNA fragmentation were examined immunohistologically after corneal infection of mice with three different kinds of HSV-2 strain 186: the wild-type virus, a US3-deficient mutant (L1BR1) and its revertant (L1B-11). In both wild-type 186- and L1B-11-infected mice, viral antigen was diffusely found in the corneal epithelium; no apoptotic changes were detected in the epithelial cells. Whereas, in L1BR1-infected mice, HSV-immunoreactivity was localized around the virus-inoculated sites, and a large number of apoptotic bodies were observed in the corneal epithelium with dual-positive reactions for both HSV-immunostaining and TUNEL staining. These results suggest that the US3 protein kinase plays an important role in protecting HSV-2-infected cells from apoptotic death in vivo.
为阐明单纯疱疹病毒2型（HSV - 2）的US3蛋白激酶在体内的生物学作用，在用三种不同的HSV - 2毒株186感染小鼠角膜后，通过免疫组织学方法检测了病毒抗原的表达、凋亡小体的出现及DNA片段化情况。这三种毒株分别为：野生型病毒、US3缺陷型突变株（L1BR1）及其回复株（L1B - 11）。在野生型186和L1B - 11感染的小鼠中，病毒抗原在角膜上皮中呈弥漫性分布；上皮细胞未检测到凋亡变化。然而，在L1BR1感染的小鼠中，HSV免疫反应性定位于病毒接种部位周围，并且在角膜上皮中观察到大量凋亡小体，其对HSV免疫染色和TUNEL染色均呈双阳性反应。这些结果表明，US3蛋白激酶在体内保护HSV - 2感染的细胞免于凋亡死亡中起重要作用。
