Ip C, Tepperman H M, Holohan P, Tepperman J
J Lipid Res. 1976 Nov;17(6):588-99.
The effect of fat feeding on adipocyte insulin binding was examined to expand a study of adaptive changes in plasma membrane functions. Cells from rats fed a high fat (L) diet for five to seven days bound less insulin and showed a decreased response to insulin (glucose oxidation) compared to those from rats fed a high glucose (G) diet. Both high and low affinity sites were influenced; the extent of the binding difference increased as increasing concentrations of insulin were present in the assay medium. Diet did not change hormone degradation on the capacity of phospholipase C to increase binding. Concanavalin A effects on fat cells were also decreased by L diet both in inhibition of insulin binding and its insulin-like effect on glucose oxidation. Spermine, which had no effect on insulin binding, also had a smaller insulin-like effect on glucose oxidation by L cells than by G cells. Serum insulin was significantly lower (30 +/- 3.7 muU/ml) in L than in G (43 +/- 3.1 muU/ml) groups. Dietary fat produces alterations in fat cells that decrease insulin binding as a part of a complex overall adaptation to the diet.
为了拓展对质膜功能适应性变化的研究，检测了高脂喂养对脂肪细胞胰岛素结合的影响。与喂食高糖（G）饮食的大鼠相比，喂食高脂肪（L）饮食五至七天的大鼠的细胞结合的胰岛素较少，并且对胰岛素（葡萄糖氧化）的反应降低。高亲和力和低亲和力位点均受到影响；随着测定培养基中胰岛素浓度的增加，结合差异的程度也增加。饮食并未改变激素降解或磷脂酶C增加结合的能力。伴刀豆球蛋白A对脂肪细胞的作用也因L饮食而降低，无论是对胰岛素结合的抑制还是其对葡萄糖氧化的胰岛素样作用。精胺对胰岛素结合没有影响，对L细胞葡萄糖氧化的胰岛素样作用也比对G细胞小。L组的血清胰岛素（30±3.7μU/ml）显著低于G组（43±3.1μU/ml）。作为对饮食复杂整体适应的一部分，饮食脂肪会导致脂肪细胞发生改变，从而降低胰岛素结合。
