Oka Y, Akanuma Y, Kasuga M, Kosaka K
Diabetologia. 1980 Nov;19(5):468-74. doi: 10.1007/BF00281828.
To elucidate the mechanisms whereby changes in dietary composition affect the action of insulin on glucose metabolism, insulin binding and glucose uptake and oxidation have been studied in epididymal fat pad adipocytes from rats fed high glucose diets for 5 and 10 days. After 5 days, insulin binding was increased, the mainly to an increased number of receptors (3.4 X 10(5) vs. 2.4 X 10(5) sites per cell) in spite of increased plasma insulin levels (3.0 +/- 0.2 vs. 2.1 +/- 0.1 microgram/l; P < 0.05). The maximal response of glucose oxidation to insulin was increased (925 +/- 55 vs. 510 +/- 58 n moles/2 X 10(5) cells/2h; P < 0.01) and the dose-response curve of glucose uptake was shifted to the left. After 10 days, receptor number decreased to the control level and the effect of insulin on glucose uptake and oxidation (% basal) were similar to controls. Thus, in the early stage of high glucose feeding, insulin receptor number, and insulin sensitivity of glucose uptake, and insulin responsiveness of glucose oxidation were increased.
为阐明饮食组成变化影响胰岛素对葡萄糖代谢作用的机制，对喂食高糖饮食5天和10天的大鼠附睾脂肪垫脂肪细胞中的胰岛素结合、葡萄糖摄取及氧化进行了研究。5天后，尽管血浆胰岛素水平升高（3.0±0.2对2.1±0.1微克/升；P<0.05），胰岛素结合增加，主要是由于受体数量增加（每个细胞3.4×10⁵对2.4×10⁵个位点）。葡萄糖氧化对胰岛素的最大反应增加（925±55对510±58纳摩尔/2×10⁵个细胞/2小时；P<0.01），葡萄糖摄取的剂量反应曲线左移。10天后，受体数量降至对照水平，胰岛素对葡萄糖摄取和氧化的作用（相对于基础水平的百分比）与对照相似。因此，在高糖喂养的早期阶段，胰岛素受体数量、葡萄糖摄取的胰岛素敏感性以及葡萄糖氧化的胰岛素反应性均增加。
