Jeremić V, Nikolić R
J Clin Chem Clin Biochem. 1976 Oct;14(10):479-83. doi: 10.1515/cclm.1976.14.1-12.479.
A simple, rapid, quantitative, low-cost UV-differential spectrophotometric method for the routine monitoring of phenobarbital in serum and cerebrospinal fluid is described. This method can also be used for monitoring methylphenobarbital (Phemiton) and primidone (Mysoline), since the major active metabolite of both drugs is phenobarbital. The basis for our procedure is Goldbaum's method (Anal. Chem., 24, 1604 (1952)). This method was improved with several modifications including a single extraction technique with dichloroethane, which has produced a very simple but still accurate method. The whole analysis takes less than 20 minutes. This procedure is accurate in the range from 1-100 mg/1 of phenobarbital in serum or cerebrospinal fluid.
本文描述了一种简单、快速、定量、低成本的紫外差示分光光度法，用于常规监测血清和脑脊液中的苯巴比妥。该方法也可用于监测甲基苯巴比妥（费米通）和扑米酮（麦苏林），因为这两种药物的主要活性代谢产物都是苯巴比妥。我们方法的基础是戈德鲍姆法（《分析化学》，第24卷，第1604页（1952年））。该方法经过了若干改进，包括用二氯乙烷进行单次萃取技术，从而产生了一种非常简单但仍然准确的方法。整个分析过程耗时不到20分钟。该方法在血清或脑脊液中苯巴比妥浓度为1 - 100毫克/升的范围内准确可靠。
