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A rapid and simple micromethod to measure picograms of norepinephrine (NE) and dopamine (DA) in small samples of brain tissue and pituitary gland.

作者信息

Zschaeck L L, Ramírez V D

出版信息

J Neural Transm. 1976;39(4):291-300. doi: 10.1007/BF01266305.

DOI:10.1007/BF01266305
PMID:993791
Abstract

A rapid and simple procedure to determine Dopamine (DA) and Norepinephrine (NE) in small tissue samples (mug quantities) is described. The radioenzymatic assay sensitivity, defined as twice the blank, is about 0.1 ng for NE and DA. Drugs known to modify brain catecholamine metabolism were shown to alter differentially DA and NE concentration in the medial basal hypothalamus (MBH) of male rats. Different areas of the brain and the pituitary gland, namely the parietal cortex, the pre-optic region (POA), the median eminence (ME), the medial basal hypothalamus without the median eminence and the posterior pituitary contain different concentrations of DA and NE, emphasizing the importance of regional analyses in tissue amines determination.

摘要

相似文献

1
A rapid and simple micromethod to measure picograms of norepinephrine (NE) and dopamine (DA) in small samples of brain tissue and pituitary gland.
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2
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3

本文引用的文献

1
Regional studies of catecholamines in the rat brain. I. The disposition of [3H]norepinephrine, [3H]dopamine and [3H]dopa in various regions of the brain.大鼠脑中儿茶酚胺的区域研究。I. [3H]去甲肾上腺素、[3H]多巴胺和[3H]多巴在脑不同区域的分布。
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The metabolism of tritiated dopamine in regions of the rat brain in vivo. I. The separation of catecholamines and their metabolites.体内大鼠脑区中氚标记多巴胺的代谢。I. 儿茶酚胺及其代谢产物的分离。
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Norepinephrine and dopamine content of hypothalamic nuclei of the rat.
A radioenzymatic method to measure picogram amounts of dopamine and 3,4-dihydroxyphenylacetic acid (DOPAC) in small samples of brain tissue.
一种用于测量脑组织小样本中皮克量多巴胺和3,4-二羟基苯乙酸（DOPAC）的放射酶法。
Experientia. 1978 Jun 15;34(6):739-41. doi: 10.1007/BF01947294.
大鼠下丘脑核中去甲肾上腺素和多巴胺的含量
Brain Res. 1974 Aug 30;77(1):137-49. doi: 10.1016/0006-8993(74)90810-5.
4
Use of catechol O-methyltransferase for the enzyme radiochemical assay of dopamine.儿茶酚O -甲基转移酶在多巴胺酶放射化学测定中的应用。
J Neurochem. 1973 Nov;21(5):1337-40. doi: 10.1111/j.1471-4159.1973.tb07587.x.
5
Letter: Catecholamines in the median eminence: new evidence for a major noradrenergic input.信件：正中隆起中的儿茶酚胺：主要去甲肾上腺素能输入的新证据。
Nature. 1973 Jun 22;243(5408):465-7. doi: 10.1038/243465a0.
6
Catecholamines in fetal and newborn rat brain.胎鼠和新生鼠脑中的儿茶酚胺
J Neurochem. 1973 Jul;21(1):61-7. doi: 10.1111/j.1471-4159.1973.tb04225.x.
7
Isolation and radioenzymic estimation of picogram quantities of dopamine and norepinephrine in biological samples.生物样品中皮克级多巴胺和去甲肾上腺素的分离及放射酶法测定
J Neurochem. 1976 Mar;26(3):471-80. doi: 10.1111/j.1471-4159.1976.tb01498.x.
8
A sensitive radioenzymatic assay for norepinephrine in tissues and plasma.一种用于检测组织和血浆中去甲肾上腺素的灵敏放射酶分析法。
Life Sci. 1975 Feb 1;16(3):375-84. doi: 10.1016/0024-3205(75)90258-1.
9
Putative neurotransmitters involved in discharging gonadotropin-releasing neurohormones and the action of LH-releasing hormone on the CNS.参与释放促性腺激素释放神经激素的假定神经递质以及促黄体生成素释放激素对中枢神经系统的作用。
Life Sci. 1975 Mar 15;16(6):833-52. doi: 10.1016/0024-3205(75)90001-6.