Konecny J, Schneider A
J Antibiot (Tokyo). 1978 Aug;31(8):776-82. doi: 10.7164/antibiotics.31.776.
Alkalimetric pH-stat titrations of cephalosporin C, cephacetril and their deacetyl derivatives using an acetyl esterase and beta-lactamase are described. The esterase was used to assay highly purified samples of cephalosporin C and cephacetril, and also to prepare analytically defined solutions of the deacetyl cephalosporins. Lactamase-catalyzed hydrolysis of the parent compounds was then found to generate exactly 2 equivalents of acid per mole; that of the deacetyl derivatives exactly 1 equivalent.
描述了使用乙酰酯酶和β-内酰胺酶对头孢菌素C、头孢乙腈及其脱乙酰衍生物进行碱量法pH滴定。酯酶用于分析高纯度的头孢菌素C和头孢乙腈样品，还用于制备脱乙酰头孢菌素的分析定义溶液。然后发现，母化合物经内酰胺酶催化水解，每摩尔恰好产生2当量的酸；脱乙酰衍生物则恰好产生1当量的酸。
