Matsuuchi L, Morrison S L
J Immunol. 1978 Sep;121(3):962-5.
Methods are described for the isolation and characterization of picogram quantities of anti-dextran antibodies. 14C-dextrans produced by using the dextransucrases of Leuconostoc mesenteroides strains B1355 and B512 were used in a radioimmunoassay. The specificity of this assay was verified by using cell cytoplasmic lysates from mouse plasmacytomas, J558 (anti-alpha 1 leads to 3 dextran) and W3129 (anti-alpha 1 leads to 6 dextran). Dextran produced by strain B1355 and insolubilized with epichlorohydrin was used as an immunoabsorbent.
本文描述了皮克级抗葡聚糖抗体的分离和特性鉴定方法。使用肠膜明串珠菌B1355和B512菌株的葡聚糖蔗糖酶产生的14C-葡聚糖用于放射免疫分析。通过使用来自小鼠浆细胞瘤J558（抗α1→3葡聚糖）和W3129（抗α1→6葡聚糖）的细胞质裂解物验证了该分析的特异性。用B1355菌株产生并用环氧氯丙烷不溶性化的葡聚糖作为免疫吸附剂。
