松鼠猴逆转录病毒前病毒序列在灵长类动物DNA中的自然分布。

Natural distribution of squirrel monkey retrovirus proviral sequences in primate DNAs.

作者信息

Schochetman G, Fine D L

出版信息

J Gen Virol. 1978 Jul;40(1):257-61. doi: 10.1099/0022-1317-40-1-257.

Abstract

3H-labelled 70S RNA of squirrel monkey retrovirus (SMRV) hybridized to a high degree (greater than 52%) to the DNA of various tissues of two squirrel monkeys. Hybridization of the same probe to DNAs of other primates including New World monkeys (Woolly monkey, capuchin, owl monkey), Old World monkeys (rhesus, African green), apes (gibbon, chimpanzee), and human (A204 cells infected with MPMV) revealed no significant hybridization. Analysis of the kinetics of hybridization indicated that SMRV provirus was present in multiple copies in various squirrel monkey tissues (C0t 1/2 = 120 to 400) and in SMRV-infected A204 cells at a low number of copies (C0t 1/2 = 1500). These results demonstrate that SMRV is an endogenous virus of squirrel monkeys and the first isolated from a New World monkey.

摘要

松鼠猴逆转录病毒（SMRV）的3H标记70S RNA与两只松鼠猴各种组织的DNA高度杂交（大于52%）。用同一探针与其他灵长类动物的DNA杂交，包括新大陆猴（绒毛猴、卷尾猴、夜猴）、旧大陆猴（恒河猴、非洲绿猴）、猿（长臂猿、黑猩猩）以及人类（感染MPMV的A204细胞），未发现明显杂交。杂交动力学分析表明，SMRV前病毒在松鼠猴的各种组织中以多拷贝形式存在（C0t 1/2 = 120至400），而在感染SMRV的A204细胞中拷贝数较低（C0t 1/2 = 1500）。这些结果表明，SMRV是松鼠猴的一种内源性病毒，也是首次从新大陆猴中分离出来的病毒。