Chiu I M, Andersen P R, Aaronson S A, Tronick S R
J Virol. 1983 Sep;47(3):434-41. doi: 10.1128/JVI.47.3.434-441.1983.
The closed circular form of the endogenous squirrel monkey type D retrovirus (SMRV) was molecularly cloned in a bacteriophage vector. The restriction map of the biologically active clone was determined and found to be identical to that of the parental SMRV linear DNA except for the deletion of one long terminal repeat. Restriction enzyme analysis and Southern blotting indicated that the SMRV long terminal repeat was approximately 300 base pairs long. The SMRV restriction map was oriented to the viral RNA by using a gene-specific probe from baboon endogenous virus. Restriction enzyme digests of a variety of vertebrate DNAs were analyzed for DNA sequence homology with SMRV by using the cloned SMRV genome as a probe. Consistent with earlier studies, multiple copies of SMRV were detected in squirrel monkey DNA. Related fragments were also detected in the DNAs from other primate species, including humans.
内源性松鼠猴D型逆转录病毒(SMRV)的闭环形式在噬菌体载体中进行了分子克隆。确定了具有生物活性的克隆的限制性图谱,发现除了缺失一个长末端重复序列外,它与亲本SMRV线性DNA的限制性图谱相同。限制性酶切分析和Southern印迹表明,SMRV长末端重复序列约为300个碱基对长。通过使用来自狒狒内源性病毒的基因特异性探针,将SMRV限制性图谱与病毒RNA进行了比对。通过使用克隆的SMRV基因组作为探针,分析了多种脊椎动物DNA的限制性酶切产物与SMRV的DNA序列同源性。与早期研究一致,在松鼠猴DNA中检测到多个SMRV拷贝。在包括人类在内的其他灵长类物种的DNA中也检测到了相关片段。