Matsubara S, Takizawa T, Sato I
Department of Obstetrics and Gynecology, Jichi Medical School, Tochigi, Japan.
Placenta. 1999 Jan;20(1):81-5. doi: 10.1053/plac.1998.0346.
The purpose of the present study was to localize glucose-6-phosphatase (G6Pase) activity in the human placenta at various gestational stages and to compare them to pre-eclamptic placenta activity. Ultrastructural enzyme-histochemical analysis of G6Pase was performed using cerium and lead as capturing agents. Precipitates indicative of G6Pase activity were observed in the endoplasmic reticulum and the nuclear envelope of the syncytiotrophoblasts in near-term placenta obtained from women with normal pregnancies. In placenta taken from women with severe pre-eclampsia, the localization pattern, enzyme activity intensity, and morphology of the endoplasmic reticulum did not differ from normal pregnancies. Stringent control experiments were performed also to ensure specific detection of G6Pase activity. The results indicate that cytochemically detectable G6Pase is present in the human placenta. This enzyme may play significant roles in carbohydrate metabolism in the human placenta.
本研究的目的是在不同妊娠阶段定位人胎盘中葡萄糖-6-磷酸酶（G6Pase）的活性，并将其与子痫前期胎盘的活性进行比较。使用铈和铅作为捕获剂对G6Pase进行超微结构酶组织化学分析。在从正常妊娠妇女获得的足月胎盘中，合体滋养层细胞的内质网和核膜中观察到了指示G6Pase活性的沉淀物。在患有严重子痫前期的妇女的胎盘中，内质网的定位模式、酶活性强度和形态与正常妊娠无差异。还进行了严格的对照实验以确保特异性检测G6Pase活性。结果表明，人胎盘中存在细胞化学可检测的G6Pase。这种酶可能在人胎盘的碳水化合物代谢中发挥重要作用。
