Watanabe J, Kanamura S, Kanai K, Shugyo Y
Anat Rec. 1986 Jan;214(1):25-31. doi: 10.1002/ar.1092140105.
Cytochemical and biochemical glucose 6-phosphatase (G6Pase) activity and fiber type composition were studied in soleus (SOL) and gastrocnemius (GC) muscles of mice. The SOL is a red muscle which contains numerous type I fibers (60%) and relatively few type II fibers (40%). The GC is a white muscle which contains numerous type II fibers (90-100%) and very few type I fibers (0-10%). In the SOL and GC, cytochemical G6Pase activity was localized in the sarcoplasmic reticulum, lateral elements of triads, myonuclear envelope, and in the endoplasmic reticulum and nuclear envelope of endothelial cells. Differential centrifugation showed that G6Pase activity was recovered in the 105,000g pellet (microsomal fraction). Histochemical enzyme activity in type II fibers was slightly higher than that in type I fibers. Biochemical G6Pase activity in the GC was significantly higher than that in the SOL. The possible functional significance of G6Pase in skeletal muscles was discussed.
对小鼠比目鱼肌（SOL）和腓肠肌（GC）的细胞化学和生化葡萄糖-6-磷酸酶（G6Pase）活性以及纤维类型组成进行了研究。比目鱼肌是一种红色肌肉，含有大量的I型纤维（60%）和相对较少的II型纤维（40%）。腓肠肌是一种白色肌肉，含有大量的II型纤维（90 - 100%）和极少的I型纤维（0 - 10%）。在比目鱼肌和腓肠肌中，细胞化学G6Pase活性定位于肌浆网、三联体的外侧成分、肌核膜以及内皮细胞的内质网和核膜。差速离心显示G6Pase活性在105,000g沉淀（微粒体部分）中得以回收。II型纤维中的组织化学酶活性略高于I型纤维。腓肠肌中的生化G6Pase活性显著高于比目鱼肌。讨论了G6Pase在骨骼肌中的可能功能意义。
