Loro L L, Vintermyr O K, Johannessen A C, Liavaag P G, Jonsson R
Department of Odontology-Oral Pathology and Forensic Odontology, Haukeland University Hospital, and University of Bergen, Norway.
J Oral Pathol Med. 1999 Feb;28(2):82-7. doi: 10.1111/j.1600-0714.1999.tb02001.x.
Apoptosis and the expression of Fas receptor (Fas) and Fas ligand (FasL) were studied in 8 samples of normal oral mucosa (OM) and in 19 oral squamous cell carcinomas (OSCC) by immunohistochemistry and the TUNEL method. Fas was detected in less than 2% of cells in OSCC compared with 84.3+/-9.0% of cells in the basal layer in OM. FasL was found to be highly expressed in poorly differentiated lesions (90.9+/-3.6%), and in cells of both the basal (88.3+/-4.3%) and central (85.3+/-5.7%) parts of moderately differentiated lesions, whereas in well-differentiated (WD) lesions expression was considerably lower in both basal (42.7+/-4.1%) and central (11.5+/-2.4%) parts. In normal OM FasL was primarily detected in cells of the basal layer, but in a high proportion of cells (84.9+/-4.3%). Apoptotic cell death was greater in OSCC (1.6+/-0.6%) than in OM (0.6+/-0.2%, P<0.05) and was most pronounced in the central part of WD OSCC (2.3+/-0.5%). Our results show that Fas is expressed in low quantities in OSCC and that FasL expression correlates negatively with degree of differentiation and apoptosis in OSCC.
通过免疫组织化学和TUNEL法，对8例正常口腔黏膜（OM）样本和19例口腔鳞状细胞癌（OSCC）进行凋亡及Fas受体（Fas）和Fas配体（FasL）表达的研究。在OSCC中，Fas在不到2%的细胞中被检测到，而在OM的基底层细胞中这一比例为84.3±9.0%。FasL在低分化病变中高表达（90.9±3.6%），在中分化病变的基底层（88.3±4.3%）和中央层（85.3±5.7%）细胞中均有高表达，而在高分化（WD）病变中，基底层（42.7±4.1%）和中央层（11.5±2.4%）的表达均显著降低。在正常OM中，FasL主要在基底层细胞中被检测到，但比例较高（84.9±4.3%）。OSCC中的凋亡细胞死亡（1.6±0.6%）高于OM（0.6±0.2%，P<0.05），且在WD OSCC的中央部分最为明显（2.3±0.5%）。我们的结果表明，Fas在OSCC中低表达，且FasL表达与OSCC的分化程度和凋亡呈负相关。
