经聚合酶链反应诊断的脉络膜结核。一例临床病理病例报告。
Choroidal tuberculosis diagnosed by polymerase chain reaction. A clinicopathologic case report.
作者信息
Bowyer J D, Gormley P D, Seth R, Downes R N, Lowe J
机构信息
Department of Ophthalmology, Queen's Medical Centre, Nottingham, England.
出版信息
Ophthalmology. 1999 Feb;106(2):290-4. doi: 10.1016/S0161-6420(99)90068-4.
PURPOSE
To show the use of the polymerase chain reaction (PCR) in a granulomatous choroidal lesion to support a diagnosis of tuberculosis.
DESIGN
Observational case report.
TESTING
Nucleic acid target amplification of a choroidal specimen using PCR for detection of Mycobacterium tuberculosis was tested.
MAIN OUTCOME MEASURES
Positive nucleic acid target amplification for M. tuberculosis in the ocular sample was measured.
RESULTS
PCR was positive for M. tuberculosis with appropriate negative controls.
CONCLUSIONS
PCR was thought to be a useful supportive technique in the diagnosis of choroidal tuberculosis.
目的
展示聚合酶链反应（PCR）在肉芽肿性脉络膜病变中用于支持结核病诊断的应用。
设计
观察性病例报告。
检测
使用PCR对脉络膜标本进行核酸靶标扩增，以检测结核分枝杆菌。
主要观察指标
测量眼样本中结核分枝杆菌核酸靶标扩增呈阳性的情况。
结果
在适当的阴性对照下，PCR检测结核分枝杆菌呈阳性。
结论
PCR被认为是诊断脉络膜结核的一种有用的辅助技术。
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