Gondim F A, da-Graça J R, de-Oliveira G R, Rêgo M C, Gondim R B, Rola F H
Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, CE, Brasil.
Braz J Med Biol Res. 1998 Dec;31(12):1605-10. doi: 10.1590/s0100-879x1998001200015.
We studied the effect of complete spinal cord transection (SCT) on gastric emptying (GE) and on gastrointestinal (GI) and intestinal transits of liquid in awake rats using the phenol red method. Male Wistar rats (N = 65) weighing 180-200 g were fasted for 24 h and complete SCT was performed between C7 and T1 vertebrae after a careful midline dorsal incision. GE and GI and intestinal transits were measured 15 min, 6 h or 24 h after recovery from anesthesia. A test meal (0.5 mg/ml phenol red in 5% glucose solution) was administered intragastrically (1.5 ml) and the animals were sacrificed by an i.v. thiopental overdose 10 min later to evaluate GE and GI transit. For intestinal transit measurements, 1 ml of the test meal was administered into the proximal duodenum through a cannula inserted into a gastric fistula. GE was inhibited (P < 0.05) by 34.3, 23.4 and 22.7%, respectively, at 15 min, 6 h and 24 h after SCT. GI transit was inhibited (P < 0.05) by 42.5, 19.8 and 18.4%, respectively, at 15 min, 6 h and 24 h after SCT. Intestinal transit was also inhibited (P < 0.05) by 48.8, 47.2 and 40.1%, respectively, at 15 min, 6 h and 24 h after SCT. Mean arterial pressure was significantly decreased (P < 0.05) by 48.5, 46.8 and 41.5%, respectively, at 15 min, 6 h and 24 h after SCT. In summary, our report describes a decreased GE and GI and intestinal transits in awake rats within the first 24 h after high SCT.
我们采用酚红法研究了完全性脊髓横断（SCT）对清醒大鼠胃排空（GE）以及胃肠道（GI）液体转运和肠内液体转运的影响。体重180 - 200 g的雄性Wistar大鼠（N = 65）禁食24小时，在仔细的中线背部切口后，于C7和T1椎骨之间进行完全性SCT。在从麻醉中恢复后15分钟、6小时或24小时测量GE、GI和肠内转运。通过胃内给予（1.5 ml）测试餐（5%葡萄糖溶液中含0.5 mg/ml酚红），10分钟后通过静脉注射过量硫喷妥钠处死动物以评估GE和GI转运。对于肠内转运测量，通过插入胃瘘的套管将1 ml测试餐注入十二指肠近端。SCT后15分钟、6小时和24小时，GE分别被抑制（P < 0.05）34.3%、23.4%和22.7%。SCT后15分钟、6小时和24小时，GI转运分别被抑制（P < 0.05）42.5%、19.8%和18.4%。SCT后15分钟、6小时和24小时，肠内转运也分别被抑制（P < 0.05）48.8%、47.2%和40.1%。SCT后15分钟、6小时和24小时，平均动脉压分别显著降低（P < 0.05）48.5%、46.8%和41.5%。总之，我们的报告描述了清醒大鼠在高位SCT后的最初24小时内GE、GI和肠内转运降低。
