de Jonge Ramon, Pot Raymond G J, Loffeld Ruud J L F, van Vliet Arnoud H M, Kuipers Ernst J, Kusters Johannes G
Department of Gastroenterology and Hepatology, Erasmus MC-University Medical Center Rotterdam, Rotterdam, The Netherlands.
Helicobacter. 2004 Apr;9(2):158-64. doi: 10.1111/j.1083-4389.2004.00213.x.
Helicobacter pylori factors that contribute to disease outcome are largely unknown, but intimate contact with host cells mediated by outer membrane proteins is thought to play an important role. Expression of the outer membrane proteins OipA, HopZ, SabA, and SabB is regulated by phase-variable dinucleotide repeats in the coding regions of the respective genes. We have evaluated the correlation between the expression status of these four genes and disease outcome of H. pylori infection in a Dutch patient population.
H. pylori strains, isolated from 96 Dutch patients with gastritis (n = 29), duodenal ulcer (n = 28), gastric ulcer (n = 21), gastric carcinoma (n = 9), and lymphoma (n = 9), were analyzed for the 'on/off' expression status of the H. pylori genes oipA, hopZ, sabA, and sabB by direct DNA sequence analysis of amplified fragments.
The off-status of sabB was significantly associated with duodenal ulcer (p =.036), but not with gastric ulcer. In contrast, the expression status of oipA, hopZ, and sabA did not correlate with disease outcome. Furthermore, lymphoma strains appeared to express a significantly smaller amount of putative adhesins when compared to gastritis, gastric ulcer, duodenal ulcer and gastric carcinoma strains (p <.02 for all groups tested).
The off-status of sabB was found to be associated with duodenal ulcer disease, and thus represents a putative marker for disease outcome. Assuming that SabB is involved in bacterial adhesion, this association suggests that adherent H. pylori are more prone to elimination by the host immune system.
幽门螺杆菌中导致疾病转归的因素大多未知，但由外膜蛋白介导的与宿主细胞的密切接触被认为起着重要作用。外膜蛋白OipA、HopZ、SabA和SabB的表达受各自基因编码区中可变二核苷酸重复序列的调控。我们在一组荷兰患者群体中评估了这四个基因的表达状态与幽门螺杆菌感染疾病转归之间的相关性。
从96名荷兰患者中分离出幽门螺杆菌菌株，这些患者分别患有胃炎（n = 29）、十二指肠溃疡（n = 28）、胃溃疡（n = 21）、胃癌（n = 9）和淋巴瘤（n = 9）。通过对扩增片段进行直接DNA序列分析，检测幽门螺杆菌基因oipA、hopZ、sabA和sabB的“开/关”表达状态。
sabB的关闭状态与十二指肠溃疡显著相关（p = 0.036），但与胃溃疡无关。相比之下，oipA、hopZ和sabA的表达状态与疾病转归无关。此外，与胃炎、胃溃疡、十二指肠溃疡和胃癌菌株相比，淋巴瘤菌株似乎表达的假定黏附素数量明显更少（所有测试组p < 0.02）。
发现sabB的关闭状态与十二指肠溃疡疾病相关，因此代表了一种疾病转归的假定标志物。假设SabB参与细菌黏附，这种关联表明黏附的幽门螺杆菌更容易被宿主免疫系统清除。
