Janas R M, Socha J, Warnawin K, Rujner J
Department of Radioimmunology, The Children's Memorial Health Institute, Warsaw, Poland.
Dig Dis Sci. 1999 Jan;44(1):170-6. doi: 10.1023/a:1026626822298.
Aim of this study was to determine and further characterize the serum aminopeptidase-M in children with liver diseases. Based on our new assay, we have shown two fractions of the enzyme. Activity of the first fraction is expressed in undiluted serum at pH adjusted from 8.5 (pH of storaged serum) to 7.4. Activity of the second fraction (cryptic activity) appears in the serum (pH 7.4) as a result of dilution and/or addition of aniline naphthalene sulfonic acid. In children with Alagille syndrome, extrahepatic biliary duct atresia, Byler's disease, and acute hepatitis due to hepatitis B virus infection, activities of both fractions are highly elevated as compared to healthy children or those with chronic viral hepatitis. Moreover, serum aminopeptidase-M seems to reflect other aspects of the pathological process than those reflected by the alanine aminotransferase and gamma-glutamyltranspeptidase. Due to increased activity and broad substrate specificity, the enzyme seems to be also a cofactor of cholestasis and hepatitis.
本研究的目的是测定并进一步表征患有肝脏疾病儿童的血清氨肽酶-M。基于我们的新检测方法，我们已显示该酶有两个组分。第一个组分的活性在未稀释血清中，pH从8.5（储存血清的pH）调节至7.4时表现出来。第二个组分的活性（隐性活性）由于稀释和/或添加苯胺萘磺酸而在血清（pH 7.4）中出现。在患有阿拉吉列综合征、肝外胆管闭锁、布勒氏病以及乙型肝炎病毒感染所致急性肝炎的儿童中，与健康儿童或患有慢性病毒性肝炎的儿童相比，两个组分的活性均显著升高。此外，血清氨肽酶-M似乎反映了病理过程中与丙氨酸转氨酶和γ-谷氨酰转肽酶所反映的不同的其他方面。由于活性增加和底物特异性广泛，该酶似乎还是胆汁淤积和肝炎的一个辅助因子。
