拟南芥内贝壳杉烯氧化酶催化赤霉素生物合成的三个步骤。
Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.
作者信息
Helliwell C A, Poole A, Peacock W J, Dennis E S
机构信息
Commonwealth Scientific and Industrial Research Organization, Plant Industry, G.P.O. Box 1600, Canberra, ACT 2601, Australia.
出版信息
Plant Physiol. 1999 Feb;119(2):507-10. doi: 10.1104/pp.119.2.507.
Abstract
The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ent-kaurene to ent-kaurenoic acid.
摘要
拟南芥GA3 cDNA在酵母（酿酒酵母）中表达，并检测了转化酵母细胞代谢贝壳杉烯的能力。我们通过全扫描气相色谱 - 质谱法表明，转化细胞产生贝壳杉烯酸，并证明单一酶GA3（贝壳杉烯氧化酶）催化从贝壳杉烯到贝壳杉烯酸的赤霉素生物合成的三个步骤。
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本文引用的文献
1
Studies on the Specificity and Site of Action of alpha-Cyclopropyl-alpha-[p-methoxyphenyl]-5-pyrimidine Methyl Alcohol (Ancymidol), a Plant Growth Regulator.α-环丙基-α-[对甲氧基苯基]-5-嘧啶甲醇（增效醚），一种植物生长调节剂的特异性和作用部位的研究。
Plant Physiol. 1978 Oct;62(4):571-6. doi: 10.1104/pp.62.4.571.
2
GIBBERELLIN BIOSYNTHESIS: Enzymes, Genes and Their Regulation.赤霉素生物合成：酶、基因及其调控
Annu Rev Plant Physiol Plant Mol Biol. 1997 Jun;48:431-460. doi: 10.1146/annurev.arplant.48.1.431.
3
Thermoinductive Regulation of Gibberellin Metabolism in Thlaspi arvense L. (II. Cold Induction of Enzymes in Gibberellin Biosynthesis).遏蓝菜中赤霉素代谢的热诱导调节（II. 赤霉素生物合成中酶的冷诱导）
Plant Physiol. 1993 Jun;102(2):547-552. doi: 10.1104/pp.102.2.547.
4
Grain Development Mutants of Barley ([alpha]-Amylase Production during Grain Maturation and Its Relation to Endogenous Gibberellic Acid Content).大麦籽粒发育突变体（籽粒成熟期间α-淀粉酶的产生及其与内源赤霉素含量的关系）
Plant Physiol. 1997 May;114(1):203-212. doi: 10.1104/pp.114.1.203.
5
Cloning of the Arabidopsis ent-kaurene oxidase gene GA3.拟南芥贝壳杉烯氧化酶基因GA3的克隆
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):9019-24. doi: 10.1073/pnas.95.15.9019.
6
Yeast expression of animal and plant P450s in optimized redox environments.在优化的氧化还原环境中动植物细胞色素P450的酵母表达
Methods Enzymol. 1996;272:51-64. doi: 10.1016/s0076-6879(96)72008-6.
7
Gibberellins: perception, transduction and responses.赤霉素：感知、转导及响应
Plant Mol Biol. 1994 Dec;26(5):1529-55. doi: 10.1007/BF00016489.
8
The maize Dwarf3 gene encodes a cytochrome P450-mediated early step in Gibberellin biosynthesis.玉米矮化3基因编码赤霉素生物合成中由细胞色素P450介导的早期步骤。
Plant Cell. 1995 Aug;7(8):1307-17. doi: 10.1105/tpc.7.8.1307.
9
Synthesis of functional mouse cytochromes P-450 P1 and chimeric P-450 P3-1 in the yeast Saccharomyces cerevisiae.在酿酒酵母中合成功能性小鼠细胞色素P-450 P1和嵌合细胞色素P-450 P3-1
Gene. 1988 May 30;65(2):203-17. doi: 10.1016/0378-1119(88)90457-x.
10
Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi.藤仓赤霉菌贝壳杉烯氧化酶活性的表征
Biochim Biophys Acta. 1990 Nov 9;1036(2):151-7. doi: 10.1016/0304-4165(90)90027-t.
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