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拟南芥内贝壳杉烯氧化酶催化赤霉素生物合成的三个步骤。

Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.

作者信息

Helliwell C A, Poole A, Peacock W J, Dennis E S

机构信息

Commonwealth Scientific and Industrial Research Organization, Plant Industry, G.P.O. Box 1600, Canberra, ACT 2601, Australia.

出版信息

Plant Physiol. 1999 Feb;119(2):507-10. doi: 10.1104/pp.119.2.507.

DOI:10.1104/pp.119.2.507
PMID:9952446
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC32127/
Abstract

The Arabidopsis GA3 cDNA was expressed in yeast (Saccharomyces cerevisiae) and the ability of the transformed yeast cells to metabolize ent-kaurene was tested. We show by full-scan gas chromatography-mass spectrometry that the transformed cells produce ent-kaurenoic acid, and demonstrate that the single enzyme GA3 (ent-kaurene oxidase) catalyzes the three steps of gibberellin biosynthesis from ent-kaurene to ent-kaurenoic acid.

摘要

拟南芥GA3 cDNA在酵母（酿酒酵母）中表达，并检测了转化酵母细胞代谢贝壳杉烯的能力。我们通过全扫描气相色谱 - 质谱法表明，转化细胞产生贝壳杉烯酸，并证明单一酶GA3（贝壳杉烯氧化酶）催化从贝壳杉烯到贝壳杉烯酸的赤霉素生物合成的三个步骤。

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