Zhang Zihan, Wu Qing-Yang, Ge Yue, Huang Zheng-Yu, Hong Ran, Li Aitao, Xu Jian-He, Yu Hui-Lei
State Key Laboratory of Bioreactor Engineering, Shanghai Collaborative Innovation Centre for Biomanufacturing, East China University of Science and Technology, Shanghai, 200237, China.
CAS Key Laboratory of Synthetic Chemistry of Natural Substances, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, China.
Bioresour Bioprocess. 2023 Jul 13;10(1):39. doi: 10.1186/s40643-023-00656-1.
Terpenoids are pervasive in nature and display an immense structural diversity. As the largest category of plant secondary metabolites, terpenoids have important socioeconomic value in the fields of pharmaceuticals, spices, and food manufacturing. The biosynthesis of terpenoid skeletons has made great progress, but the subsequent modifications of the terpenoid framework are poorly understood, especially for the functionalization of inert carbon skeleton usually catalyzed by hydroxylases. Hydroxylase is a class of enzymes that plays an important role in the modification of terpenoid backbone. This review article outlines the research progress in the identification, molecular modification, and functional expression of this class of enzymes in the past decade, which are profitable for the discovery, engineering, and application of more hydroxylases involved in the plant secondary metabolism.
萜类化合物在自然界中广泛存在，结构极为多样。作为植物次生代谢产物中最大的一类，萜类化合物在制药、香料和食品制造领域具有重要的社会经济价值。萜类骨架的生物合成已取得了很大进展，但萜类骨架随后的修饰却知之甚少，尤其是对于通常由羟化酶催化的惰性碳骨架的功能化修饰。羟化酶是一类在萜类骨架修饰中起重要作用的酶。本文综述了过去十年中这类酶在鉴定、分子修饰和功能表达方面的研究进展，这将有助于发现、改造和应用更多参与植物次生代谢的羟化酶。
