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猪DNA的序列组织

Sequence organization of porcine DNA.

作者信息

Avvedimento V E, Acquaviva A M, Varrone S

出版信息

Nucleic Acids Res. 1976 Oct;3(10):2491-505. doi: 10.1093/nar/3.10.2491.

DOI:10.1093/nar/3.10.2491
PMID:995641
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343108/
Abstract

The sequence organization of porcine DNA isolated from thyroid has been analyzed by hydroxylapatite (HAP) chromatography. The reassociation of 0.4 kilobase (Kb) DNA fragments shows, besides the presence of 5% inverted repeat sequences (foldback DNA), that 45% of the genome is represented by high (10%) and intermediate (35%) repetitive components, whereas the remaining 50% is unique sequences. 30% of the unique sequences consists of 1,000 nucleotide fragments interspersed with repetitive elements 400 nucleotides in length. The remaining 20% is longer unique sequences (10,000 nucleotides) apparently not linked to repetitive elements.

摘要

通过羟基磷灰石（HAP）色谱法分析了从甲状腺分离出的猪DNA的序列组织。0.4千碱基（Kb）DNA片段的复性显示，除了存在5%的反向重复序列（回文DNA）外，基因组的45%由高度（10%）和中度（35%）重复成分组成，而其余50%是单拷贝序列。30%的单拷贝序列由1000个核苷酸的片段组成，这些片段散布着长度为400个核苷酸的重复元件。其余20%是明显与重复元件不相关的较长单拷贝序列（10000个核苷酸）。

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本文引用的文献

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Sequence organization of the human genome.人类基因组的序列组织
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Interspersion of repetitive and nonrepetitive DNA sequences in the Drosophila melanogaster genome.果蝇基因组中重复和非重复DNA序列的散布情况。
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