圆二色性作为重组核组蛋白内部DNA结构的探针。
Circular dichroism as a probe of DNA structure inside reconstituted nucleohistones.
作者信息
Garel A, Kovacs A M, Champagne M, Daune M
出版信息
Nucleic Acids Res. 1976 Oct;3(10):2507-19. doi: 10.1093/nar/3.10.2507.
Abstract
Reconstituted nucleohistones were obtained by mixing in given conditions acid extracted histones and eukaryotic DNA. The histone/DNA ratio (w/w) was in the range 0.35 - 0.95. With the four histones (H2A2B) we have been able to obtain subunits (nucleosomes or upsilon-bodies). The variation of cirsular dichroism signal with temperature at 280 nm was measured to follow structural changes of the DNA inside the complex. The true change of ellipticity (see article) of histone-bound DNA regions, is similar for reconstituted nucleohistone and H1-depleted chromatin, and is therefore a physical probe of the presence of nucleosomes.
摘要
通过在特定条件下将酸提取的组蛋白与真核生物DNA混合，获得了重组核组蛋白。组蛋白与DNA的比例（重量/重量）在0.35 - 0.95范围内。利用四种组蛋白（H2A2B），我们能够获得亚基（核小体或υ体）。在280nm处测量圆二色性信号随温度的变化，以跟踪复合物内部DNA的结构变化。重组核组蛋白和H1缺失染色质中与组蛋白结合的DNA区域的椭圆率真实变化（见文章）相似，因此是核小体存在的物理探针。
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