Sulkowski S
Department of Pathological Anatomy, Medical Academy of Białystok.
Rocz Akad Med Bialymst. 1998;43:56-65.
The aim of the present study was to analyse the effect of single intraperitoneal administration of cyclophosphamide (150 mg/kg b.w.) on the morphological changes in erythrocytes. The study of erythrocytes was based on the ultrastructural analysis in the transmission electron microscope (TEM)--in situ examinations and ultrastructural analysis in the scanning electron microscope (SEM)--in vitro examinations of the blood collected from the right and left ventricle of the heart. A single intraperitoneal cyclophosphamide administration was found to cause in the deformability of erythrocytes, which was observed both in TEM and SEM. The altered shape of erythrocytes observed in SEM referred mainly to the blood samples collected from the left ventricle rather than the right. The results obtained suggest that the changes in erythrocyte deformability may cause the development of pulmonary microembolism observed after intraperitoneal administration of cyclophosphamide.
本研究的目的是分析腹腔注射一次环磷酰胺（150毫克/千克体重）对红细胞形态变化的影响。对红细胞的研究基于透射电子显微镜（TEM）的超微结构分析——原位检查，以及扫描电子显微镜（SEM）的超微结构分析——对从心脏左右心室采集的血液进行体外检查。发现单次腹腔注射环磷酰胺会导致红细胞变形性发生变化，这在TEM和SEM中均有观察到。在SEM中观察到的红细胞形状改变主要涉及从左心室而非右心室采集的血样。所得结果表明，红细胞变形性的变化可能导致腹腔注射环磷酰胺后观察到的肺微栓塞的发生。
