Sulkowski S, Sulkowska M, Musiatowicz B
Department of Pathological Anatomy, University Medical School of Bialystok, Poland.
Histol Histopathol. 1998 Oct;13(4):1027-36. doi: 10.14670/HH-13.1027.
Cyclophosphamide (CP), an antineoplastic and immunosuppressive agent, even when administered in large doses, slightly affects the quantity of blood platelets. The aim of the present study was to analyse the effect of single intraperitoneal administration of CP (150 mg/kg b.w.) on the quantitative changes in platelets obtained from the left and right ventricle of the heart, as well as to evaluate the occurrence of megakaryocytes in lung tissue depending on the period of time that passed from CP administration. In control subgroups, fewer platelets were found in the blood collected from the RV compared with the left ventricle at all time intervals. After 1 and 3 days following i.p. administration of CP, a decrease was observed in the number of platelets both in the blood from the right ventricle and left ventricle when compared with control. However, after 14 days, the number of platelets in the blood from the left ventricle was higher, compared with the left ventricle and right ventricle of control animals, and significantly higher (p < 0.001747), compared with their number obtained from the right ventricle of CP-receiving animals. Simultaneous ultrastructural examinations with transmission electron microscopy revealed the increased number of platelets in the lung vascular bed of CP-receiving rats at all time intervals. However, megakaryocytes were found 7 and 14 days after administration of CP. The findings clearly indicate that the lungs could be a major place of thrombopoiesis following therapy with a single large dose of CP.
环磷酰胺（CP）是一种抗肿瘤和免疫抑制药物，即使大剂量给药，对血小板数量的影响也较小。本研究的目的是分析单次腹腔注射CP（150mg/kg体重）对从心脏左右心室获取的血小板数量变化的影响，并根据CP给药后的时间评估肺组织中巨核细胞的出现情况。在对照亚组中，在所有时间间隔内，从右心室采集的血液中发现的血小板比左心室少。腹腔注射CP后1天和3天，与对照组相比，右心室和左心室血液中的血小板数量均减少。然而，14天后，与对照动物的左心室和右心室相比，左心室血液中的血小板数量更高，与接受CP的动物右心室获得的血小板数量相比，显著更高（p<0.001747）。同时，透射电子显微镜的超微结构检查显示，在所有时间间隔内，接受CP的大鼠肺血管床中的血小板数量增加。然而，在CP给药后7天和14天发现了巨核细胞。这些发现清楚地表明，单次大剂量CP治疗后，肺可能是血小板生成的主要部位。
