Maruyama K, Yamamoto H, Uchiyama T
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University, Japan.
Biosci Biotechnol Biochem. 1998 Nov;62(11):2223-5. doi: 10.1271/bbb.62.2223.
Pectin constituents, which were about 70 w/w% of extracellular polysaccharides (ECP) from a cell-suspension culture of Mentha, were purified by gel filtration chromatography, and their sugar composition and linkage were investigated. Two major constituents identified were (1-->3)-linked galactan carrying arabinosyl residues on C-6 and (1-->4)-alpha-linked galacturonan partially interspersed with (1-->2)-linked rhamnosyl resides. Acetylated or methylated pectins were not identified on 1H-NMR analysis.
从薄荷细胞悬浮培养物中提取的果胶成分约占细胞外多糖(ECP)的70%(重量/重量),通过凝胶过滤色谱法进行纯化,并对其糖组成和连接方式进行了研究。鉴定出的两种主要成分是在C-6位带有阿拉伯糖基残基的(1→3)连接的半乳聚糖和部分穿插有(1→2)连接的鼠李糖基残基的(1→4)-α-连接的半乳糖醛酸聚糖。在1H-NMR分析中未鉴定出乙酰化或甲基化的果胶。