Maruyama K, Goto C, Numata M, Suzuki T, Nakagawa Y, Hoshino T, Uchiyama T
Oleochemical Research Laboratory, Japan.
Phytochemistry. 1996 Mar;41(5):1309-14. doi: 10.1016/0031-9422(95)00739-3.
Extracellular polysaccharide produced by suspension-cultured Mentha cells consisted of 50% neutral sugars, 32% uronic acid and 10% of protein. The ammonium oxalate-soluble fraction of this polysaccharide contained 30% hemicellulose and 70% pectic substances. The purified hemicellulose contained xylose, glucose, arabinose, galactose, mannose and fucose residues in a molar ratio of 41.6:31.3:13.1:11.1:1.3:1.6. It was identified as a xyloglucan from its neutral sugar composition and by methylation analysis and cellulase treatment: the principal neutral sugar was arabinose. The presence of O-acetyl residues was confirmed with 1H NMR, 13C NMR and GC-mass spectrometry. The total acetyl content in the polysaccharide was 4%. The point of attachment of the O-acetyl residue was shown to be at position 6 of the galactosyl residue.
悬浮培养的薄荷细胞产生的细胞外多糖由50%的中性糖、32%的糖醛酸和10%的蛋白质组成。该多糖的草酸铵可溶部分含有30%的半纤维素和70%的果胶物质。纯化的半纤维素含有木糖、葡萄糖、阿拉伯糖、半乳糖、甘露糖和岩藻糖残基，其摩尔比为41.6:31.3:13.1:11.1:1.3:1.6。根据其中性糖组成、甲基化分析和纤维素酶处理，鉴定为木葡聚糖：主要中性糖为阿拉伯糖。通过1H NMR、13C NMR和GC-质谱法证实了O-乙酰基残基的存在。多糖中的总乙酰含量为4%。O-乙酰基残基的连接点显示在半乳糖基残基的6位。
