Arenas M I, Madrid J F, Bethencourt F R, Fraile B, Paniagua R
Department of Cell Biology and Genetics, University of Alcalá, Alcalá de Henares, Madrid, Spain.
Int J Androl. 1998 Dec;21(6):332-42. doi: 10.1046/j.1365-2605.1998.00130.x.
The oligosaccharide sequences of glycoconjugates and the nature of the saccharide linkage were investigated in normal human testes by means of lectin histochemistry studies, at light and electron microscopy levels. Reaction to WGA was intense in the seminiferous epithelium and interstitium. MAA showed light reactivity in all cell types of the human seminiferous epithelium, the lamina propria and Leydig cells. UEA-I lectin labelled the lamina propria intensely and the seminiferous epithelium and Leydig cells slightly. A slight reaction to AAA was found in the seminiferous epithelium and in Leydig cells. ConA was labelled in Sertoli cells, germ cells and Leydig cells. The reaction to GNA lectin was similar although less intense. PNA labelling was slight in Sertoli cells, spermatogonia, and Leydig cells, and more intense in spermatocytes, spermatids and peritubular cells. Reaction to DSA was intense in the seminiferous epithelium and Leydig cells. HPA labelled all cell types in the seminiferous epithelium and Leydig cells slightly, and labelled peritubular cells intensely. SBA lectin showed a strong reaction in spermatids and a slight reaction in the lamina propria. The reactions to SNA, LTA, and DBA were negative in all testicular cell types. After beta-elimination pre-treatment, MAA, UEA-I, AAA, PNA, DSA, HPA and SBA reactions were all negative. Endo F/PNGase digestion suppressed reactivity to ConA y GNA. Staining for WGA decreased with Endo F/PNGase digestion and also after beta-elimination. Desialization increased reactivity to PNA, SBA and HPA lectins. These results indicate that the terminal sequences of oligosaccharide side-chains in spermatocytes and, principally, in spermatids are: fucose, mannose, Neu5Ac2,3Gal1,3GalNAc, Gal1,3GalNAc, Gal1,4GlcNAc, Neu5AcGalNAc and GalNAc (in O-glycosylated proteins); mannose (in N-glycosylated proteins) and GlcNAc (in both protein types). A sialic acid residue is added to galactose and GalNAc residues. Present findings also indicate that Sertoli cell glycoproteins are similar to those of spermatids, and that the terminal sugar residues in Leydig cells are GlcNAc, fucose, mannose, Neu5Ac2,3Gal1,3GalNAc, Gal1,3GalNAc, and Gal1,4GlcNAc. The lectin pattern of the lamina propria suggests the presence of GlcNAc, galactose, fucose and GalNAc.
通过凝集素组织化学研究，在光学和电子显微镜水平上，对正常人类睾丸中糖缀合物的寡糖序列和糖链连接性质进行了研究。生精上皮和间质对WGA反应强烈。MAA在人类生精上皮、固有层和睾丸间质细胞的所有细胞类型中显示出轻度反应。UEA-I凝集素强烈标记固有层，轻度标记生精上皮和睾丸间质细胞。在生精上皮和睾丸间质细胞中发现对AAA有轻微反应。ConA在支持细胞、生殖细胞和睾丸间质细胞中被标记。对GNA凝集素的反应相似，但强度较低。PNA在支持细胞、精原细胞和睾丸间质细胞中标记轻微，在精母细胞、精子细胞和管周细胞中标记较强。对DSA的反应在生精上皮和睾丸间质细胞中强烈。HPA轻度标记生精上皮和睾丸间质细胞的所有细胞类型，强烈标记管周细胞。SBA凝集素在精子细胞中显示出强烈反应，在固有层中显示出轻微反应。对SNA、LTA和DBA的反应在所有睾丸细胞类型中均为阴性。经过β-消除预处理后，MAA、UEA-I、AAA、PNA、DSA、HPA和SBA反应均为阴性。内切糖苷酶F/PNG酶消化抑制了对ConA和GNA的反应性。用内切糖苷酶F/PNG酶消化以及β-消除后，WGA染色减弱。去唾液酸化增加了对PNA、SBA和HPA凝集素的反应性。这些结果表明，精母细胞以及主要是精子细胞中寡糖侧链的末端序列为：岩藻糖、甘露糖、Neu5Ac2,3Gal1,3GalNAc、Gal1,3GalNAc、Gal1,4GlcNAc、Neu5AcGalNAc和GalNAc（在O-糖基化蛋白中）；甘露糖（在N-糖基化蛋白中）和GlcNAc（在两种蛋白类型中）。唾液酸残基添加到半乳糖和GalNAc残基上。目前的研究结果还表明，支持细胞糖蛋白与精子细胞的糖蛋白相似，睾丸间质细胞中的末端糖残基为GlcNAc、岩藻糖、甘露糖、Neu5Ac2,3Gal1,3GalNAc、Gal1,3GalNAc和Gal1,4GlcNAc。固有层的凝集素模式表明存在GlcNAc、半乳糖、岩藻糖和GalNAc。
