Cook A M, Jones J G, Lane I F, Evans S A
School of Molecular and Medical Biosciences, University of Wales, Cardiff, UK.
Clin Hemorheol Microcirc. 1998 Dec;19(4):271-80.
Leukocytes are characterised from their influence on the filterability of undiluted blood from patients with PAOD (intermittent claudicants - Fontaine Stage II) and a group of sex and age-matched controls. Undiluted blood was filtered through 5 microm Nuclepore (Hemafil) filters for 300 s, at 711 Pa and room temperature, using a custom-made constant pressure filtrometer. Four populations of leukocytes are identified in both groups. In the control group, 94.4% of the leukocytes are identified as fast leukocytes with a transit time of 1.8 s. The remaining white cells are recognised as slow flowing leukocytes and subdivided into three further sub-populations. The first of these (SL1; 2.8% of total leukocyte count) is characterised by a transit time of 31.7 s, a second population (SL2; 1.5% of total leukocyte count) by a transit time of 145.8 s while the remaining cells are identified as pore blockers (PB) under these conditions. A similar rheological classification is valid in the patients but the sum of the three minor populations is elevated compared to controls (p = 0.001) although there is no overall leukocytosis. The only significant difference in flow properties of any blood cells, between the two groups of volunteers, is seen in the major population of leukocytes with an elevated transit time of 2.4 s. Stepwise regression analysis identifies the concentration of fast leukocytes, SL2 and PB as the major variables affecting blood flow through the filter. It is argued that the higher concentration of SL2 and PB probably reflect the increased sensitivity of neutrophils to physical stimuli.
通过对患有外周动脉疾病（间歇性跛行患者 - Fontaine II期）的患者以及一组性别和年龄匹配的对照组的未稀释血液的过滤性影响来表征白细胞。使用定制的恒压滤器，在711 Pa和室温下，将未稀释的血液通过5微米的核孔（Hemafil）滤器过滤300秒。在两组中均鉴定出四类白细胞。在对照组中，94.4%的白细胞被鉴定为快速白细胞，通过时间为1.8秒。其余白细胞被识别为缓慢流动的白细胞，并进一步细分为三个亚群。其中第一个亚群（SL1；占白细胞总数的2.8%）的特征是通过时间为31.7秒，第二个亚群（SL2；占白细胞总数的1.5%）的通过时间为145.8秒，而其余细胞在这些条件下被识别为孔堵塞细胞（PB）。在患者中也有类似的流变学分类，但与对照组相比，三个次要亚群的总和升高（p = 0.001），尽管没有总体白细胞增多。两组志愿者之间任何血细胞流动特性的唯一显著差异在于主要白细胞群体，其通过时间延长至2.4秒。逐步回归分析确定快速白细胞、SL2和PB的浓度是影响血液通过滤器流动的主要变量。有人认为，SL2和PB浓度较高可能反映了中性粒细胞对物理刺激的敏感性增加。
