Cook A M, Evans S A, Lane I F, Jones J G
School of Molecular and Medical Biosciences, University of Wales, Cardiff.
Br J Haematol. 1998 Sep;102(4):952-6. doi: 10.1046/j.1365-2141.1998.00878.x.
Red blood cells and about 95% of white blood cells have an immediate and constant effect on the flow of undiluted or diluted blood through 5 microm filters. The remaining 5% of all leucocytes exert an increasing influence on flow such that the rate of flow of diluted and undiluted blood through these filters is continually declining over a period of 150 s. Analysis of this declining flow rate enables these cells to be counted and their rheological properties to be deduced. Approximately 50% of these slow leucocytes pass through the filters with a transit time of about 30 s and the remaining cells act as pore blockers for 150 s. The numbers and flow properties of slow leucocytes was approximately the same in blood from young women (25 years) and older men (65 years). However, the number of slow leucocytes was increased in a group of men (65 years) suffering from peripheral arterial occlusive disease. Dilution of the blood with phosphate-buffered saline increased the numbers of slow leucocytes in both of the older, but not the younger, group of volunteers. This effect was particularly noticeable in the patient group. It is recommended that filtration studies of the rheological profile of leucocytes can, and must, be performed with undiluted blood. The properties after dilution may sometimes, but not invariably, reflect changes ex vivo as well as inherent differences in the cells themselves.
红细胞和大约95%的白细胞对未稀释或稀释后的血液通过5微米过滤器的流动有直接且持续的影响。所有白细胞中其余5%对血流的影响逐渐增加，以至于在150秒的时间段内，稀释和未稀释血液通过这些过滤器的流速持续下降。对这种下降流速的分析能够对这些细胞进行计数并推断其流变学特性。大约50%的这些缓慢白细胞以约30秒的通过时间通过过滤器，其余细胞在150秒内充当孔隙阻滞剂。年轻女性（25岁）和老年男性（65岁）血液中缓慢白细胞的数量和流动特性大致相同。然而，一组患有外周动脉闭塞性疾病的男性（65岁）中缓慢白细胞的数量增加。用磷酸盐缓冲盐水稀释血液会增加老年志愿者组（而非年轻志愿者组）中缓慢白细胞的数量。这种效应在患者组中尤为明显。建议对白细胞流变学特征的过滤研究能够且必须使用未稀释血液进行。稀释后的特性有时（但并非总是）能够反映体外变化以及细胞自身的内在差异。
