Miyamoto Y, Kim S U
Department of Medicine, University of British Columbia, Vancouver, Canada.
J Neurosci Res. 1999 Jan 15;55(2):245-51. doi: 10.1002/(SICI)1097-4547(19990115)55:2<245::AID-JNR12>3.0.CO;2-Q.
Macrophage inflammatory protein-1alpha (MIP-1alpha) is a member of a superfamily of inflammatory cytokines termed chemokines, and it has been implicated in the pathogenesis of several human diseases with inflammatory components. It has been known that MIP-1alpha plays a role in recruiting and activating mononuclear phagocytes in the central nervous system (CNS), and that astrocytes and microglia are sources of this chemokine. However, details of the regulation of MIP-1alpha production by these glial cells are not known. In the present study, expression of MIP-1alpha was determined in purified cultures of human astrocyte. MIP-1alpha mRNA levels in human astrocyte cell preparations were determined by reverse transcription polymerase chain reaction (RT-PCR) and amount of MIP-1alpha protein secreted into culture supernatants by human astrocytes was assayed by enzyme-linked immunosorbent assay (ELISA). Under the unstimulated conditions, human astrocytes did not express MIP-1alpha message or protein, indicating that human astrocytes do not constitutively carry MIP-1alpha message. Following treatment with interleukin-1beta (IL-1beta), human astrocytes demonstrated increased message and protein expression for MIP-1alpha, while other immune modulators such as interferon-gamma (IFN)-gamma, tumor necrosis factor-alpha (TNF-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), lipopolysaccharide, or phorbol ester (a protein kinase C activator) did not induce MIP-1alpha expression in human astrocytes.
巨噬细胞炎性蛋白-1α（MIP-1α）是被称为趋化因子的炎性细胞因子超家族的一员，它与几种具有炎症成分的人类疾病的发病机制有关。已知MIP-1α在中枢神经系统（CNS）中募集和激活单核吞噬细胞方面发挥作用，并且星形胶质细胞和小胶质细胞是这种趋化因子的来源。然而，这些神经胶质细胞对MIP-1α产生的调节细节尚不清楚。在本研究中，在人星形胶质细胞的纯化培养物中测定了MIP-1α的表达。通过逆转录聚合酶链反应（RT-PCR）测定人星形胶质细胞制剂中的MIP-1α mRNA水平，并通过酶联免疫吸附测定（ELISA）测定人星形胶质细胞分泌到培养上清液中的MIP-1α蛋白量。在未刺激的条件下，人星形胶质细胞不表达MIP-1α信息或蛋白，这表明人星形胶质细胞不组成性携带MIP-1α信息。在用白细胞介素-1β（IL-1β）处理后，人星形胶质细胞显示出MIP-1α的信息和蛋白表达增加，而其他免疫调节剂如干扰素-γ（IFN）-γ、肿瘤坏死因子-α（TNF-α）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、脂多糖或佛波酯（一种蛋白激酶C激活剂）在人星形胶质细胞中不诱导MIP-1α表达。
