Lillard J W, Boyaka P N, Hedrick J A, Zlotnik A, McGhee J R
Department of Microbiology and Immunobiology Vaccine Center, University of Alabama, Birmingham 35294, USA.
J Immunol. 1999 Feb 15;162(4):1959-65.
Lymphotactin (Lptn) is a C chemokine produced predominantly by NK and CD8-positive (CD8+) T cells including gammadelta TCR-positive (TCR+) intraepithelial lymphocytes. Lptn is chemotactic for NK and T cells and likely plays an important role in maintaining the integrity of the epithelium and in mucosal immune responses. In this study, we characterized the immune responses to OVA given intranasally with Lptn to mice. This regimen enhanced OVA-specific serum Ab responses and Ab titers in mucosal secretions. Lptn also enhanced OVA-specific Ab-forming cells in mucosal and systemic compartments. CD4-positive (CD4+) T cells isolated from mucosal compartments and spleens of mice intranasally immunized with OVA plus Lptn displayed higher OVA-specific proliferative responses and greater synthesis of IFN-gamma, IL-2, IL-4, IL-5, IL-6, and IL-10 than did CD4+ T cells from mice given OVA without Lptn. These studies indicate that Lptn has adjuvant properties and suggest that Lptn present in the mucosa has the potential to enhance mucosal and systemic Ab responses through help provided by Th1- and Th2-type cells to link the initial innate signals of the mucosa with the acquired immune system.
淋巴细胞趋化因子（Lptn）是一种C类趋化因子，主要由自然杀伤细胞（NK）和CD8阳性（CD8 +）T细胞产生，包括γδT细胞受体阳性（TCR +）上皮内淋巴细胞。Lptn对NK细胞和T细胞具有趋化作用，可能在维持上皮完整性和黏膜免疫反应中发挥重要作用。在本研究中，我们对用Lptn经鼻给予小鼠卵清蛋白（OVA）后的免疫反应进行了特征分析。该方案增强了OVA特异性血清抗体反应以及黏膜分泌物中的抗体滴度。Lptn还增强了黏膜和全身区室中OVA特异性抗体形成细胞。从经鼻用OVA加Lptn免疫的小鼠的黏膜区室和脾脏中分离出的CD4阳性（CD4 +）T细胞，与未用Lptn给予OVA的小鼠的CD4 + T细胞相比，表现出更高的OVA特异性增殖反应以及更高水平的γ干扰素、白细胞介素-2、白细胞介素-4、白细胞介素-5、白细胞介素-6和白细胞介素-10合成。这些研究表明Lptn具有佐剂特性，并提示黏膜中存在的Lptn有可能通过Th1型和Th2型细胞提供的辅助作用来增强黏膜和全身抗体反应，从而将黏膜的初始固有信号与获得性免疫系统联系起来。
