Hartas J, Sriprakash K S
Menzies School of Health Research, Darwin, Casuarina NT, Australia.
Microb Pathog. 1999 Jan;26(1):25-33. doi: 10.1006/mpat.1998.0244.
Streptococcus pyogenes infection and acute glomerulonephritis (AGN), a non-suppurtave disease, are endemic in the Aboriginal people of the Northern Territory (NT) of Australia. Vir typing, a locus-specific polymerase chain reaction (PCR)-based typing method [Gardiner, Hartas, Currie et al PCR Meth Appl 1995 4: 288-93], has revealed high divergence among the NT streptococcal strains. A total of 76 Vir types (VTs) representing about 95% of the NT isolates were screened for sic, a gene for streptococcal inhibitor of complement function, by PCR and hybridization. This revealed that seven VTs are positive for sic, and there are two classes of the gene: those closely related to sic (CRS) originally described by Akesson, Sjoholm & Bjorck [ J. Biol. Chem. 1996 271: 1081-8] and those distantly related to sic (DRS). Among the CRS-positive VTs, VT16, VT78 and VT91 have emm (gene for M protein) encoding type 1 M protein or related specificity, and VT8 and VT101 contain emm57 or related alleles. Chromosomal location of CRS in emm57 is different from that in emm1 or related strains. The DRS-positive VT18 and VT52 contained emm55 and emm12 respectively, which are phylogenetically related. Strains of S. pyogenes types 1, 12, 55 and 57 are known to be associated with AGN. Restricted distribution of CRS and DRS among the M types historically associated with AGN suggests that these sic alleles may have a role in AGN pathogenesis.
化脓性链球菌感染与急性肾小球肾炎（AGN），一种非化脓性疾病，在澳大利亚北领地（NT）的原住民中呈地方性流行。病毒分型，一种基于位点特异性聚合酶链反应（PCR）的分型方法[加德纳、哈塔斯、柯里等人，《PCR方法与应用》1995年第4期：288 - 93页]，已揭示NT链球菌菌株之间存在高度差异。通过PCR和杂交，对总共76种代表约95%的NT分离株的病毒型（VTs）进行了补体功能链球菌抑制剂基因sic的筛选。结果显示，七种VTs对sic呈阳性，并且该基因有两类：一类与阿克松、肖霍尔姆和比约克最初描述的sic密切相关（CRS）[《生物化学杂志》1996年第271卷：1081 - 1088页]，另一类与sic远缘相关（DRS）。在CRS阳性的VTs中，VT16、VT78和VT91具有编码1型M蛋白或相关特异性的emm（M蛋白基因），而VT8和VT101含有emm57或相关等位基因。emm57中CRS的染色体定位与emm1或相关菌株中的不同。DRS阳性的VT18和VT52分别含有emm55和emm12，它们在系统发育上相关。已知化脓性链球菌1型、12型、55型和57型菌株与AGN有关。CRS和DRS在历史上与AGN相关的M型中分布受限，这表明这些sic等位基因可能在AGN发病机制中起作用。
