Angellis D, Inglis N R, Fishman W H
Am J Clin Pathol. 1976 Dec;66(6):929-34. doi: 10.1093/ajcp/66.6.929.
The examination of alkaline phosphatase isoenzymes by means of isoelectric focusing in polyacrylamide gel rods using the apparatus and focusing method of Righetti and Drysdale is discussed. A simultaneous coupling procedure using alpha-naphthyl phosphate and fast blue salt R in 2-amino-2-methyl-1,3-propanediol buffer, pH 9.68, containing MgCl2 and ZnSO4 proved sensitive for developing the enzyme bands. Also discussed are the effects seen with the incorporation of Triton X100 into the gel and sample mixtures. Enzyme, which remained at the top of the gel without using this detergent, entered the gel easily with the addition of Triton X-100 into the application solution. Incorporation of Triton into the gel matrix resulted in some enzyme band patterns that showed distinct differences from gels containing no Triton.
讨论了使用里盖蒂（Righetti）和德赖斯代尔（Drysdale）的仪器及聚焦方法，通过在聚丙烯酰胺凝胶棒中进行等电聚焦来检测碱性磷酸酶同工酶。在含有氯化镁（MgCl2）和硫酸锌（ZnSO4）的pH 9.68的2-氨基-2-甲基-1,3-丙二醇缓冲液中，使用磷酸α-萘酯和固蓝盐R的同步偶联程序被证明对显影酶带很灵敏。还讨论了在凝胶和样品混合物中加入聚山梨醇酯X100（Triton X100）所观察到的效果。在不使用这种去污剂的情况下，酶保留在凝胶顶部，而在加样溶液中加入Triton X-100后，酶很容易进入凝胶。将Triton加入凝胶基质中会导致一些酶带图谱与不含Triton的凝胶显示出明显差异。
