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[猪精液颗粒大小及个体特性与解冻后活动精子数量的关系]

[Size of pellets and individual properties of boar semen in relation to the number of motile spermatozoa following defrosting].

作者信息

Kozumplík J

出版信息

Vet Med (Praha). 1978 Aug;23(8):457-63.

PMID:99858
Abstract

The second fraction of the ejaculate of five boars of the Landrace breed was used for the experiments. Non-diluted semen was kept in hydrogen atmosphere for three hours at + 5 degrees C; then it was frozen in solid carbon dioxide into 0.1 ml, 2.0 ml, and 5.0 ml pellets and in liquid nitrogen vapours into the shape of disk or cylinder 5 ml in volume. The pellets were de-frozen on a teflon pan at 42 degrees C without addition of any other diluent. Ejaculate in which at least 25% of the spermatozoa were motile was considered to be usable. No statistically significant difference was found between the motility of spermatozoa frozen in the 0.1 ml and 2.0 ml pellets, but it can be said that on an average the motility of the spermatozoa was lower in larger pellets; this could be observed mainly in the pellets with the volume of 5.0 ml. Large differences were revealed in the freezability of the semen of different boars, irrespective of the size of the frozen pellet. Insemination of 38 gilts with semen from 2.0 ml pellets gave a 57,8 % concentration rate (22 gilts) and the average litter size was 8.25 piglets.

摘要

选用五头长白猪品种公猪射精的第二部分用于实验。未稀释的精液在5摄氏度的氢气环境中保存3小时;然后在固态二氧化碳中冷冻成0.1毫升、2.0毫升和5.0毫升的颗粒,并在液氮蒸气中冷冻成体积为5毫升的圆盘或圆柱形状。颗粒在42摄氏度的特氟龙盘上解冻,不添加任何其他稀释剂。精子活力至少为25%的射精被认为可用。在0.1毫升和2.0毫升颗粒中冷冻的精子活力之间未发现统计学上的显著差异,但可以说,平均而言,较大颗粒中精子的活力较低;这主要在体积为5.0毫升的颗粒中观察到。不同公猪精液的冷冻能力存在很大差异,与冷冻颗粒的大小无关。用来自2.0毫升颗粒的精液对38头后备母猪进行授精,受孕率为57.8%(22头后备母猪),平均产仔数为8.25头仔猪。

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