Peña F J, Saravia F, Núñez-Martínez I, Johannisson A, Wallgren M, Rodriguez Martinez H
Animal Reproduction and Obstetrics, Faculty of Veterinary Medicine, University of Extremadura, Avda de la Universidad s/n, 10071 Cáceres, Spain.
Anim Reprod Sci. 2006 Jun;93(1-2):101-13. doi: 10.1016/j.anireprosci.2005.06.028. Epub 2005 Aug 9.
Previous studies have shown sperm quality post-cryopreservation differs depending on the fraction of the seminal plasma boar spermatozoa are fortuitously contained in. As such, spermatozoa contained in the first 10 mL of the sperm-rich fraction (portion I) have better sustained handling procedures (extension, handling and freezing/thawing) than those contained in the ulterior part of a fractionated ejaculate (second portion of the sperm-rich fraction and the post-spermatic fraction, portion II). However, those studies were performed using pooled samples. In the present study, individual ejaculates were used. Split ejaculates (portions I and II) from five boars were frozen and thawed using a conventional freezing protocol, followed by computer-assisted motility and morphology analysis (CASA and ASMA, respectively), as well as an Annexin-V assay for spermatozoa from each boar and ejaculate portion. Significant differences between portions were observed in all ASMA-derived variables, except in one boar. Also significant differences were observed between boars and ejaculate portions in sperm quality post-thaw. We identified, however, boars showing best results of motility and sperm membrane integrity post-thaw in portion I, while in other boar the best results was observed in portion II. It is concluded that the identification of the ejaculate portion more suitable to sustain cryopreservation in each individual boar may be a readily applicable and easy technique to diminish variation in sperm freezability among boars.
先前的研究表明，冷冻保存后的精子质量因公猪精液中偶然包含的精浆部分不同而有所差异。因此，富含精子部分的前10毫升（部分I）中所含的精子，比分级射精的后续部分（富含精子部分的第二部分和精子后部分，部分II）中所含的精子，在处理程序（稀释、处理和冷冻/解冻）上具有更好的耐受性。然而，这些研究是使用混合样本进行的。在本研究中，使用的是个体射精样本。从五头公猪采集的分割射精样本（部分I和部分II），采用传统冷冻方案进行冷冻和解冻，随后分别进行计算机辅助运动分析（CASA）和精子形态分析（ASMA），以及对每头公猪和射精部分的精子进行膜联蛋白-V检测。除一头公猪外，在所有ASMA衍生变量中均观察到部分之间存在显著差异。在解冻后的精子质量方面，公猪和射精部分之间也观察到显著差异。然而，我们发现，部分I中解冻后运动性和精子膜完整性表现最佳的公猪，而在其他公猪中，部分II的结果最佳。得出的结论是，确定每头公猪中更适合承受冷冻保存的射精部分，可能是一种易于应用且简便的技术，可减少公猪间精子冷冻能力的差异。
