Martin J C, Klug E, Günzel A R
J Reprod Fertil Suppl. 1979(27):47-51.
In a study of different methods of handling stallion semen for deep freezing, ejaculates were divided into 3 portions, the first of which was diluted 1:2 with lactose--egg yolk--glycerol diluent and frozen in pellet form. The second aliquot was centrifuged without any diluent and the third portion was initially diluted with an experimental diluent (Merck) and then centrifuged for 5 min at 1000 g. The second and third portions were frozen in large volume straws each of which contained one whole insemination dose of 1 or 2 X 10(8) progressively motile spermatozoa. The addition of a diluent to the semen before centrifugation and freezing (portion 3) resulted in an increase in sperm motility after thawing. Motility was further increased by the use of a recently developed diluent after centrifugation and before freezing. In one fertility trial, 12 of 19 mares (63%) conceived following a single insemination of frozen semen during one oestrous period.
在一项关于冷冻保存种马精液不同方法的研究中，射精精液被分成3份，第一份用乳糖 - 蛋黄 - 甘油稀释液按1:2稀释并制成颗粒冷冻。第二份等分试样不添加任何稀释剂进行离心，第三份先用一种实验性稀释剂（默克公司产品）稀释，然后以1000g离心5分钟。第二份和第三份被冷冻在大容量细管中，每个细管含有一整剂授精剂量的1或2×10⁸条进行性运动精子。在离心和冷冻前向精液中添加稀释剂（第三份）可使解冻后精子活力增加。在离心后和冷冻前使用一种新开发的稀释剂可进一步提高精子活力。在一项受精试验中，19匹母马中有12匹（63%）在一个发情期单次输精冷冻精液后受孕。
