Chanbancherd P, Brown A E, Trichavaroj R, Tienamporn P, Puthakird P, Limpairojn N, VanCott T C, de Souza M S
Army Institute of Pathology, Bangkok, Thailand.
J Clin Microbiol. 1999 Mar;37(3):804-6. doi: 10.1128/JCM.37.3.804-806.1999.
Dried blood spot (DBS) specimens were assessed as an alternative to plasma for human immunodeficiency virus type 1 (HIV-1) serotyping by V3 loop peptide enzyme immunoassay. Nested PCR capable of distinguishing HIV-1 subtypes B and E was used as the reference standard. Ninety-two percent of DBS samples were typeable as either HIV-1 subtype B or E. Serotype results with DBS and plasma were identical for 254 of 257 specimens. A simple DBS collection method provides a convenient alternative for conducting HIV-1 serotype surveillance while retaining sensitivity and specificity.
通过V3环肽酶免疫测定法,对干血斑(DBS)标本作为人类免疫缺陷病毒1型(HIV-1)血清分型血浆替代物进行了评估。能够区分HIV-1 B亚型和E亚型的巢式PCR用作参考标准。92%的DBS样本可分型为HIV-1 B亚型或E亚型。257份标本中的254份,DBS和血浆的血清型结果相同。一种简单的DBS采集方法为开展HIV-1血清型监测提供了一种方便的替代方法,同时保留了敏感性和特异性。