Basso G, Timeus F
Dipartimento di Scienze Pediatriche, Università di Torino, Italy.
Bone Marrow Transplant. 1998 Dec;22 Suppl 5:S17-20.
The cytofluorimetric enumeration of CD34-positive cells is a useful method for measuring haematopoietic stem cells. The large variation of results between different laboratories, in multicenter quality control studies, could be due to multiple technical problems. CD34 analysis must be performed in whole blood and the NH4Cl or the NH4Cl-derived reagents give the best results in the lysis procedure. The monoclonal antibody for CD34 detection must be a class III, if possible PE-conjugated. Any differences were found in the absolute CD34 count using different analysis protocols: Milan, ISHAGE, Dutch, or a commercial kit, when the sample is fresh and in good viable condition. The multiparametric approach, using three or four colours simultaneously, could be required in scoring apheresis products from patients with CD34-positive blast cells (ie ALL of B cell origin) and in deciding the optimum time to start the leukapheresis, choosing, with an equal CD34 number, the moment at which the highest number of more immature progenitor cells (CD38-negative or dim, CD90-positive) is present.
CD34阳性细胞的细胞荧光计数是一种用于测量造血干细胞的有用方法。在多中心质量控制研究中，不同实验室之间结果的巨大差异可能是由于多种技术问题。CD34分析必须在全血中进行，NH4Cl或NH4Cl衍生试剂在裂解过程中效果最佳。用于检测CD34的单克隆抗体必须是III类，如有可能应与PE偶联。当样本新鲜且活力良好时，使用不同的分析方案（米兰方案、ISHAGE方案、荷兰方案或商业试剂盒）在绝对CD34计数上未发现任何差异。对于来自CD34阳性原始细胞（即B细胞来源的急性淋巴细胞白血病）患者的单采产品进行评分以及确定开始白细胞单采的最佳时间时，可能需要采用同时使用三种或四种颜色的多参数方法，在CD34数量相同的情况下，选择存在更多未成熟祖细胞（CD38阴性或弱阳性、CD90阳性）数量最多的时刻。
