使用新型干细胞计数试剂盒对脐血中的有活力 CD34+细胞进行计数。
Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit.
机构信息
Department of Laboratory Medicine, Shanghai General Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.
Department of Hematology and Oncology, Shanghai Children's Medical Center, Shanghai JiaoTong University School of Medicine, Shanghai, China.
出版信息
J Int Med Res. 2021 Nov;49(11):3000605211055384. doi: 10.1177/03000605211055384.
OBJECTIVE
To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences.
METHODS
Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were enumerated using a BD FACSCanto instrument and FACSDiva software.
RESULTS
A total of 519 samples were analyzed in this study. The hematopoietic stem cell enumeration kit developed by BD Biosciences yielded absolute counts of CD34-positive cells that were on average 8.7% lower than Beckman Coulter Stem-Kit reagents (range: -5.7% to-14.7%). The BD Biosciences kit yielded relative counts that were on average 9.9% higher compared with Beckman Coulter Stem-Kit reagents (range: -2.1% to +13.8%). The intraclass correlation coefficients for absolute and relative counts of CD34-positive cells were 0.9967 (95% confidence interval [CI]: 0.9961-0.9972) and 0.9512 (95% CI: 0.9423-0.9587) for the BD Biosciences and Beckman Coulter kits, respectively.
CONCLUSIONS
The hematopoietic stem cell enumeration kit developed by BD Biosciences can be used to enumerate CD34-positive stem cells from cord blood samples.
目的
评估 BD Biosciences 开发的造血干细胞计数试剂盒的检测性能。
方法
使用 BD Biosciences 开发的造血干细胞计数试剂盒和 Beckman Coulter 的 Stem-Kit 试剂制备脐带血样本。使用 BD FACSCanto 仪器和 FACSDiva 软件计数 CD34+细胞。
结果
本研究共分析了 519 个样本。BD Biosciences 开发的造血干细胞计数试剂盒得出的 CD34 阳性细胞绝对计数平均比 Beckman Coulter Stem-Kit 试剂低 8.7%（范围：-5.7%至-14.7%）。BD Biosciences 试剂盒得出的相对计数平均比 Beckman Coulter Stem-Kit 试剂高 9.9%（范围：-2.1%至+13.8%）。BD Biosciences 和 Beckman Coulter 试剂盒的 CD34 阳性细胞绝对计数和相对计数的组内相关系数分别为 0.9967（95%置信区间[CI]：0.9961-0.9972）和 0.9512（95% CI：0.9423-0.9587）。
结论
BD Biosciences 开发的造血干细胞计数试剂盒可用于计数脐带血样本中的 CD34 阳性干细胞。
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