• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用新型干细胞计数试剂盒对脐血中的有活力 CD34+细胞进行计数。

Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit.

机构信息

Department of Laboratory Medicine, Shanghai General Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, China.

Department of Hematology and Oncology, Shanghai Children's Medical Center, Shanghai JiaoTong University School of Medicine, Shanghai, China.

出版信息

J Int Med Res. 2021 Nov;49(11):3000605211055384. doi: 10.1177/03000605211055384.

DOI:10.1177/03000605211055384
PMID:34775864
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8593314/
Abstract

OBJECTIVE

To assess the detection performance of the hematopoietic stem cell enumeration kit developed by BD Biosciences.

METHODS

Cord blood samples were prepared using a hematopoietic stem cell enumeration kit developed by BD Biosciences and Stem-Kit reagents from Beckman Coulter. CD34+ cells were enumerated using a BD FACSCanto instrument and FACSDiva software.

RESULTS

A total of 519 samples were analyzed in this study. The hematopoietic stem cell enumeration kit developed by BD Biosciences yielded absolute counts of CD34-positive cells that were on average 8.7% lower than Beckman Coulter Stem-Kit reagents (range: -5.7% to-14.7%). The BD Biosciences kit yielded relative counts that were on average 9.9% higher compared with Beckman Coulter Stem-Kit reagents (range: -2.1% to +13.8%). The intraclass correlation coefficients for absolute and relative counts of CD34-positive cells were 0.9967 (95% confidence interval [CI]: 0.9961-0.9972) and 0.9512 (95% CI: 0.9423-0.9587) for the BD Biosciences and Beckman Coulter kits, respectively.

CONCLUSIONS

The hematopoietic stem cell enumeration kit developed by BD Biosciences can be used to enumerate CD34-positive stem cells from cord blood samples.

摘要

目的

评估 BD Biosciences 开发的造血干细胞计数试剂盒的检测性能。

方法

使用 BD Biosciences 开发的造血干细胞计数试剂盒和 Beckman Coulter 的 Stem-Kit 试剂制备脐带血样本。使用 BD FACSCanto 仪器和 FACSDiva 软件计数 CD34+细胞。

结果

本研究共分析了 519 个样本。BD Biosciences 开发的造血干细胞计数试剂盒得出的 CD34 阳性细胞绝对计数平均比 Beckman Coulter Stem-Kit 试剂低 8.7%（范围：-5.7%至-14.7%）。BD Biosciences 试剂盒得出的相对计数平均比 Beckman Coulter Stem-Kit 试剂高 9.9%（范围：-2.1%至+13.8%）。BD Biosciences 和 Beckman Coulter 试剂盒的 CD34 阳性细胞绝对计数和相对计数的组内相关系数分别为 0.9967（95%置信区间[CI]：0.9961-0.9972）和 0.9512（95% CI：0.9423-0.9587）。

结论

BD Biosciences 开发的造血干细胞计数试剂盒可用于计数脐带血样本中的 CD34 阳性干细胞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/1bb7b04aa909/10.1177_03000605211055384-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/bd912406e30a/10.1177_03000605211055384-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/53c170f50df4/10.1177_03000605211055384-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/884f516c4213/10.1177_03000605211055384-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/5ecae8846a6d/10.1177_03000605211055384-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/1bb7b04aa909/10.1177_03000605211055384-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/bd912406e30a/10.1177_03000605211055384-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/53c170f50df4/10.1177_03000605211055384-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/884f516c4213/10.1177_03000605211055384-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/5ecae8846a6d/10.1177_03000605211055384-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3550/8593314/1bb7b04aa909/10.1177_03000605211055384-fig5.jpg

相似文献

1
Enumeration of viable CD34+ cells in cord blood using a novel stem cell enumeration kit.使用新型干细胞计数试剂盒对脐血中的有活力 CD34+细胞进行计数。
J Int Med Res. 2021 Nov;49(11):3000605211055384. doi: 10.1177/03000605211055384.
2
Multi-site evaluation of the BD Stem Cell Enumeration Kit for CD34(+) cell enumeration on the BD FACSCanto II and BD FACSCalibur flow cytometers.使用BD FACSCanto II和BD FACSCalibur流式细胞仪，对BD干细胞计数试剂盒进行多中心评估，用于CD34(+)细胞计数。
Cytotherapy. 2014 Nov;16(11):1558-1574. doi: 10.1016/j.jcyt.2014.03.006. Epub 2014 Jun 11.
3
Evaluation of the BD Stem Cell Enumeration Kit on the BD FACSCanto II flow cytometer using bd facscanto clinical and bd facsdiva software.使用 BD FACSCanto II 流式细胞仪和 bd facsdiva 软件评估 BD Stem Cell Enumeration Kit。
Int J Lab Hematol. 2021 Feb;43(1):61-67. doi: 10.1111/ijlh.13315. Epub 2020 Sep 15.
4
Enumeration of viable CD34(+) cells by flow cytometry in blood, bone marrow and cord blood: results of a study of the novel BD™ stem cell enumeration kit.流式细胞术检测血液、骨髓和脐血中活 CD34(+)细胞：新型 BD™ 干细胞计数试剂盒研究结果。
Cytotherapy. 2011 Apr;13(4):449-58. doi: 10.3109/14653249.2010.529894. Epub 2010 Nov 15.
5
Validation of a single-platform method for hematopoietic CD34+ stem cells enumeration according to accreditation procedure.根据认证程序对一种用于造血CD34+干细胞计数的单平台方法进行验证。
Biomed Mater Eng. 2015;25(1 Suppl):27-39. doi: 10.3233/BME-141226.
6
Comparison of two single-platform ISHAGE-based CD34 enumeration protocols on BD FACSCalibur and FACSCanto flow cytometers.基于ISHAGE的两种单平台CD34计数方案在BD FACSCalibur和FACSCanto流式细胞仪上的比较。
Cytotherapy. 2009;11(5):595-605. doi: 10.1080/14653240902923161.
7
A new single-platform method for the enumeration of CD34+ cells.一种新的用于计数 CD34+ 细胞的单平台方法。
Cytotherapy. 2009;11(6):804-6, 806.e1. doi: 10.3109/14653240903092628.
8
Performance of a novel BD stem cell enumeration kit on two flow cytometry systems.新型 BD 干细胞计数试剂盒在两种流式细胞仪系统上的性能。
Int J Lab Hematol. 2013 Aug;35(4):393-9. doi: 10.1111/ijlh.12029. Epub 2012 Nov 28.
9
Enumeration of CD34+ cells in cord blood: a variation on a single-platform flow cytometric method based on the ISHAGE gating strategy.脐血中CD34+细胞的计数：基于ISHAGE设门策略的单平台流式细胞术方法的一种变体
Cytometry. 2001 Aug 15;46(4):254-61. doi: 10.1002/cyto.1136.
10
CD34+ cell enumeration by flow cytometry: a comparison of systems and methodologies.流式细胞术检测 CD34+ 细胞：系统与方法的比较。
Arch Pathol Lab Med. 2011 Jul;135(7):909-14. doi: 10.5858/2010-0119-0AR.1.

本文引用的文献

1
Validation of the single-platform ISHAGE protocol for enumeration of CD34+ hematopoietic stem cells in umbilical cord blood in a Brazilian center.单平台ISHAGE方案在巴西某中心用于脐带血中CD34+造血干细胞计数的验证
Hematol Transfus Cell Ther. 2022 Jan-Mar;44(1):49-55. doi: 10.1016/j.htct.2020.09.151. Epub 2020 Dec 4.
2
Enumerating regulatory T cells in cryopreserved umbilical cord blood samples using FOXP3 methylation specific quantitative PCR.采用 FOXP3 甲基化特异性定量 PCR 技术对冷冻保存的脐血样本中的调节性 T 细胞进行计数。
PLoS One. 2020 Oct 23;15(10):e0240190. doi: 10.1371/journal.pone.0240190. eCollection 2020.
3
Evaluation of post-thaw CFU-GM: clinical utility and role in quality assessment of umbilical cord blood in patients receiving single unit transplant.
冻存后 CFU-GM 的评估：在接受单个单位移植的患者中，对脐血临床应用的评估和质量控制的作用。
Transfusion. 2020 Jan;60(1):144-154. doi: 10.1111/trf.15592. Epub 2019 Nov 22.
4
Not just a marker: CD34 on human hematopoietic stem/progenitor cells dominates vascular selectin binding along with CD44.不仅仅是一个标志物：人类造血干/祖细胞上的CD34与CD44共同主导血管选择素结合。
Blood Adv. 2017 Dec 26;1(27):2799-2816. doi: 10.1182/bloodadvances.2017004317.
5
Banking of Hematopoietic Stem Cells: Influence of Storage Time on Their Quality Parameters.
Transplant Proc. 2016 Jun;48(5):1806-9. doi: 10.1016/j.transproceed.2016.03.011.
6
Phase I/II Trial of StemRegenin-1 Expanded Umbilical Cord Blood Hematopoietic Stem Cells Supports Testing as a Stand-Alone Graft.StemRegenin-1扩增脐带血造血干细胞的I/II期试验支持将其作为独立移植物进行测试。
Cell Stem Cell. 2016 Jan 7;18(1):144-55. doi: 10.1016/j.stem.2015.10.004. Epub 2015 Dec 5.
7
Improving Quality and Potency Testing for Umbilical Cord Blood: A New Perspective.提高脐带血质量和效能检测：一个新视角。
Stem Cells Transl Med. 2015 Sep;4(9):967-73. doi: 10.5966/sctm.2015-0036. Epub 2015 Jul 9.
8
Validation of a single-platform method for hematopoietic CD34+ stem cells enumeration according to accreditation procedure.根据认证程序对一种用于造血CD34+干细胞计数的单平台方法进行验证。
Biomed Mater Eng. 2015;25(1 Suppl):27-39. doi: 10.3233/BME-141226.
9
Multi-site evaluation of the BD Stem Cell Enumeration Kit for CD34(+) cell enumeration on the BD FACSCanto II and BD FACSCalibur flow cytometers.使用BD FACSCanto II和BD FACSCalibur流式细胞仪，对BD干细胞计数试剂盒进行多中心评估，用于CD34(+)细胞计数。
Cytotherapy. 2014 Nov;16(11):1558-1574. doi: 10.1016/j.jcyt.2014.03.006. Epub 2014 Jun 11.
10
Performance of a novel BD stem cell enumeration kit on two flow cytometry systems.新型 BD 干细胞计数试剂盒在两种流式细胞仪系统上的性能。
Int J Lab Hematol. 2013 Aug;35(4):393-9. doi: 10.1111/ijlh.12029. Epub 2012 Nov 28.