南极嗜冷菌莫拉克斯氏菌属TAE123中依赖NAD(+)的乙醇脱氢酶的氢转移立体特异性
Stereospecificity of hydrogen transfer by the NAD(+)-linked alcohol dehydrogenase from the Antarctic psychrophile Moraxella sp. TAE123.
作者信息
Velonia K, Tsigos I, Bouriotis V, Smonou I
机构信息
Department of Chemistry, University of Crete, Greece.
出版信息
Bioorg Med Chem Lett. 1999 Jan 4;9(1):65-8. doi: 10.1016/s0960-894x(98)00678-7.
PMID:9990458
Abstract
Investigation of the stereochemistry of the hydride transfer in reactions catalyzed by the recently isolated NAD(+)-linked alcohol dehydrogenase from the Antarctic psychrophile Moraxella sp. TAE123 was accomplished by using 1H NMR spectroscopy of the deuterated coenzyme. It was found that this new psychrophilic enzyme is a type A dehydrogenase. Moraxella sp. ADH reduces stereospecifically 2-butanone to produce (S)-2-butanol.
摘要
通过使用氘代辅酶的1H NMR光谱法，对最近从南极嗜冷菌莫拉克斯氏菌属TAE123中分离出的NAD(+)连接的醇脱氢酶催化的反应中氢化物转移的立体化学进行了研究。结果发现，这种新的嗜冷酶是一种A型脱氢酶。莫拉克斯氏菌属ADH立体特异性地将2-丁酮还原为(S)-2-丁醇。
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