Efremenko V I
Zh Mikrobiol Epidemiol Immunobiol. 1978 Apr(4):45-9.
A new method of concentration and purification developed allows to obtain choleragen and choleragenoid preparations, homogeneous in the immunochemical analysis with regard to the culturing of vibrios producing these substances on the available nonsynthetic nutrient medium. Crystalline choleragen and choleragenoid preparations were obtained from highly purified samples. Choleragen possessed enterotoxic and skin premeability factors; choleragenoid shows no biological activity.
所开发的一种新的浓缩和纯化方法能够获得霍乱毒素和类霍乱毒素制剂，就这些物质是在现有的非合成营养培养基上培养产生它们的弧菌而言，这些制剂在免疫化学分析中是均质的。从高度纯化的样品中获得了结晶霍乱毒素和类霍乱毒素制剂。霍乱毒素具有肠毒素和皮肤通透性因子；类霍乱毒素无生物学活性。
