Silveira S R, Hadler W A
Acta Histochem. 1978;61(2):223-8. doi: 10.1016/S0065-1281(78)80069-5.
As an attempt to test the specificity of an histochemical technique proposed to detect catalases, an investigation was carried out by immunochemical techniques. Purified catalases were used after analysed immunochemically by double immune diffusion test and immunoelectrophoretic technique. These pure catalases induced, after injecting into guinea-pigs, anti-serums that react specifically with catalase and does not give any cross reaction with peroxidases and haemic iron containing compounds. By the direct and indirect immuno-fluorescence techniques it was shown an intense catalase reactivity inside the cytoplasm of adrenal cortex and hepatic cells, that appears as a granular pattern. These results are very similar to those provided by the histochemical technique, either concerning to the reactive cells or to the granular pattern of the positive reaction. In such instances, the immunochemical results suggest the specificity of the histochemical reaction. This specificity is confirmed by the previous treatment of tissue sections by catalases anti-serum. After this treatment either the immunochemical or the histochemical technique to detect catalases provide negative results on cells that before the treatment were strongly reactive.
为了测试一种用于检测过氧化氢酶的组织化学技术的特异性，采用免疫化学技术进行了一项研究。纯化的过氧化氢酶经双向免疫扩散试验和免疫电泳技术进行免疫化学分析后使用。将这些纯过氧化氢酶注射到豚鼠体内后，诱导产生了能与过氧化氢酶特异性反应且与过氧化物酶和含铁血红素的化合物无任何交叉反应的抗血清。通过直接和间接免疫荧光技术显示，肾上腺皮质和肝细胞的细胞质内存在强烈的过氧化氢酶反应性，呈颗粒状模式。这些结果与组织化学技术所提供的结果非常相似，无论是关于反应细胞还是阳性反应的颗粒状模式。在这种情况下，免疫化学结果表明了组织化学反应的特异性。通过用过氧化氢酶抗血清对组织切片进行预处理，证实了这种特异性。经过这种处理后，无论是免疫化学技术还是组织化学技术，在处理前反应强烈的细胞上检测过氧化氢酶均得到阴性结果。
