通过凝胶过滤从大分子中去除结合配体。
Removal of a bound ligand from a macromolecule by gel filtration.
作者信息
Dixion H B
出版信息
Biochem J. 1976 Oct 1;159(1):161-2. doi: 10.1042/bj1590161.
Abstract
If a solution of macromolecule of concentration c and volume a, initially saturated with a ligand of dissociation constant K, is gel filtered through a volume v of solution initially free of ligand, the saturation y with which it emerges is given by the equation y-lny-1=Kv/ca.
摘要
如果一种浓度为c、体积为a且最初被解离常数为K的配体饱和的大分子溶液，通过最初不含配体的体积为v的溶液进行凝胶过滤，那么其流出时的饱和度y由方程y - lny - 1 = Kv/ca给出。
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