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相互作用体系的小区域凝胶色谱法：动力学控制的大分子 - 配体反应洗脱曲线的理论与实验评估

Small zone gel chromatography of interacting systems: theoretical and experimental evaluation of elution profiles for kinetically controlled macromolecule-ligand reactions.

作者信息

Cann J R, York E J, Stewart J M, Vera J C, Maccioni R B

机构信息

Department of Biochemistry/Biophysics/Genetics, University of Colorado Health Sciences Center, Denver 80262.

出版信息

Anal Biochem. 1988 Dec;175(2):462-73. doi: 10.1016/0003-2697(88)90570-2.

DOI:10.1016/0003-2697(88)90570-2
PMID:3071186
Abstract

A phenomenological theory of small zone gel chromatography is elaborated for kinetically controlled macromolecule-ligand interactions. Chromatography, direct binding experiments, and rate measurements are used for successful comparison of experimental behavior with theoretical elution profiles for the interaction of MAP-2 with two synthetic peptide fragments from the C-terminal moieties of alpha- and beta-tubulin subunits. The results of this study provide guidelines for interpretation of experimental small zone elution profiles of total ligand.

摘要

针对动力学控制的大分子-配体相互作用，阐述了一种小区域凝胶色谱的现象学理论。利用色谱法、直接结合实验和速率测量，成功地将实验行为与微管相关蛋白2（MAP-2）与α-和β-微管蛋白亚基C末端部分的两个合成肽片段相互作用的理论洗脱曲线进行了比较。本研究结果为解释总配体的实验小区域洗脱曲线提供了指导。

相似文献

1
Small zone gel chromatography of interacting systems: theoretical and experimental evaluation of elution profiles for kinetically controlled macromolecule-ligand reactions.相互作用体系的小区域凝胶色谱法：动力学控制的大分子 - 配体反应洗脱曲线的理论与实验评估
Anal Biochem. 1988 Dec;175(2):462-73. doi: 10.1016/0003-2697(88)90570-2.
2
Numerical and experimental demonstrations of the need for caution in the use of zonal gel chromatography for characterizing ligand interactions with small acceptors.关于在使用区域凝胶色谱法表征配体与小受体相互作用时需谨慎的数值和实验论证。
Arch Biochem Biophys. 1989 Apr;270(1):173-83. doi: 10.1016/0003-9861(89)90019-2.
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New approach for studying macromolecular-ligand binding. Determination of the dissociation constant for macromolecule-bound ligand by gel filtration.研究大分子-配体结合的新方法。通过凝胶过滤法测定大分子结合配体的解离常数。
J Biol Chem. 1980 Apr 10;255(7):3062-7.
4
Frontal gel chromatography of interacting systems: theoretical and experimental evaluation of the shapes of elution profiles for systems of the type A + B in equilibrium C.相互作用体系的前沿凝胶色谱法：A + B ⇌ C 型体系洗脱曲线形状的理论与实验评估
Arch Biochem Biophys. 1987 Jul;256(1):78-89. doi: 10.1016/0003-9861(87)90427-9.
5
Hummel-Dreyer gel chromatographic procedure as applied to ligand-mediated association.应用于配体介导缔合的胡默尔-德雷尔凝胶色谱法。
Biochemistry. 1976 Oct 19;15(21):4614-22. doi: 10.1021/bi00666a011.
6
Analysis of protein-protein interaction by simulation of small-zone size exclusion chromatography. Stochastic formulation of kinetic rate contributions to observed high-performance liquid chromatography elution characteristics.通过小区域尺寸排阻色谱模拟分析蛋白质-蛋白质相互作用。对观察到的高效液相色谱洗脱特性的动力学速率贡献的随机公式化。
Biophys J. 1989 Jun;55(6):1155-67. doi: 10.1016/S0006-3495(89)82912-1.
7
Evaluation of nonideality from gel chromatographic partition coefficients. A technique with greater versatility than equilibrium dialysis.从凝胶色谱分配系数评估非理想性。一种比平衡透析更具通用性的技术。
Biophys Chem. 1989 May;33(2):115-25. doi: 10.1016/0301-4622(89)80014-6.
8
Scanning molecular sieve chromatography of interacting protein systems. Simulation of large zone behavior for self-associating solutes undergoing rapid chemical equilibration under kinetic control.相互作用蛋白质系统的扫描分子筛色谱法。在动力学控制下，对经历快速化学平衡的自缔合溶质的大区域行为进行模拟。
Biophys Chem. 1978 Jan;7(4):347-66. doi: 10.1016/0301-4622(78)85011-x.
9
Ligand binding and self-association of proteins.蛋白质的配体结合与自缔合
Mol Cell Biochem. 1980 May 28;31(1):5-24. doi: 10.1007/BF00817887.
10
Binding of n-mers to one-dimensional lattices with longer than close-contact interactions.具有长于紧密接触相互作用的n聚体与一维晶格的结合。
Biophys Chem. 1987 Jul;27(1):59-65. doi: 10.1016/0301-4622(87)80046-7.

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Structural evidence for cooperative microtubule stabilization by Taxol and the endogenous dynamics regulator MAP4.紫杉醇与内源性动力学调节因子 MAP4 协同稳定微管的结构证据。
ACS Chem Biol. 2012 Apr 20;7(4):744-52. doi: 10.1021/cb200403x. Epub 2012 Feb 6.
2
Analysis of protein-protein interaction by simulation of small-zone size exclusion chromatography. Stochastic formulation of kinetic rate contributions to observed high-performance liquid chromatography elution characteristics.通过小区域尺寸排阻色谱模拟分析蛋白质-蛋白质相互作用。对观察到的高效液相色谱洗脱特性的动力学速率贡献的随机公式化。
Biophys J. 1989 Jun;55(6):1155-67. doi: 10.1016/S0006-3495(89)82912-1.
3
The antineoplastic agent estramustine and the derivative estramustine-phosphate inhibit secretion of interleukin-3 in leukemic cells. Possible roles of MAPs.
抗肿瘤药物雌莫司汀及其衍生物磷酸雌莫司汀可抑制白血病细胞中白细胞介素-3的分泌。微管相关蛋白的可能作用。
Mol Cell Biochem. 1992 Nov 18;117(2):165-73. doi: 10.1007/BF00230756.