Larkins B A, Jones R A, Tsai C Y
Biochemistry. 1976 Dec 14;15(25):5506-11. doi: 10.1021/bi00670a014.
Zein messenger RNA was isolated from membrane-bound polyribosomes of developing maize kernels by oligo(dT)-cellulose chromatography. Translation of the mRNA in vitro yielded protein similar to native zein in amino acid content, ethanol solubility, and mobility on sodium dodecyl sulfate- polyacrylamide gels. The zein mRNA sedimented as a homogeneous peak on sucrose gradients and contained a poly(A)-rich region based upon hybridization to [3H]poly(U). The mRNA had an apparent molecular weight of 540 000 on agarose-acrylamide gels. It synthesized both 21 800 and 19 000 molecular weight zein components in the wheat-germ cell-free protein synthesis system. The possibility of a polycistronic mRNA or two mRNAs of similar molecular weight is considered.
通过寡聚（dT）-纤维素色谱法从发育中的玉米籽粒的膜结合多核糖体中分离出玉米醇溶蛋白信使核糖核酸（mRNA）。该mRNA在体外翻译产生的蛋白质，其氨基酸含量、乙醇溶解性以及在十二烷基硫酸钠-聚丙烯酰胺凝胶上的迁移率均与天然玉米醇溶蛋白相似。玉米醇溶蛋白mRNA在蔗糖梯度上以单一峰沉降，基于与[3H]聚尿苷（poly(U)）杂交显示其含有富含多聚腺苷酸（poly(A)）的区域。在琼脂糖-丙烯酰胺凝胶上，该mRNA的表观分子量为540000。在小麦胚芽无细胞蛋白质合成系统中，它合成了分子量为21800和19000的玉米醇溶蛋白组分。文中考虑了多顺反子mRNA或两个分子量相似的mRNA的可能性。
