Weinand U, Brüschke C, Feix G
Nucleic Acids Res. 1979 Jun 25;6(8):2707-15. doi: 10.1093/nar/6.8.2707.
Starting from polysomal RNA of developing maize endosperm and applying the cloning procedure of integrating dsDNA into the Pst I site of plasmid pBR 322, clones containing sequences complementary to endosperm mRNAs were obtained. 25 per cent of these clones were identified as containing zein specific DNA sequences which hybridized either with the zein mRNA coding for the 22 000 Mr protein or with the zein mRNA coding for the 19 000 Mr protein. The zein-specific DNA inserts of the recombinant plasmids were further characterized by restriction enzyme analysis.
从发育中的玉米胚乳的多聚核糖体RNA开始，应用将双链DNA整合到质粒pBR 322的Pst I位点的克隆程序，获得了含有与胚乳mRNA互补序列的克隆。这些克隆中有25%被鉴定为含有玉米醇溶蛋白特异性DNA序列，它们可与编码22000道尔顿蛋白质的玉米醇溶蛋白mRNA或与编码19000道尔顿蛋白质的玉米醇溶蛋白mRNA杂交。通过限制性酶切分析对重组质粒的玉米醇溶蛋白特异性DNA插入片段进行了进一步表征。
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